WT1基因表达对K562、HL-60细胞增殖作用的研究

目的：探讨ＷＴ１基因反义ＲＮＡ对髓系白血病细胞生长增殖和细胞凋亡的影响及作用机制。方法：用ＷＴ１基因反义ＲＮＡ培养Ｋ５６２、ＨＬ６０细胞，用氮蓝四氮唑盐染色、流式细胞仪检测、逆转录聚合酶链反应等方法观察细胞增殖、凋亡、细胞动力学和基因表达情况。结果：ＷＴ１反义ＲＮＡ可以特异性抑制Ｋ５６２细胞增殖，诱导Ｋ５６２、ＨＬ６０细胞凋亡；对ＨＬ６０细胞的增殖无影响，对ＷＴ１、ｂｃｌ２、ｍｄｍ２基因表达无显著影响。结论：ＷＴ１基因与白血病细胞的增殖、凋亡密切相关，对白血病细胞凋亡的影响与ｐ５３、ｂｃｌ２基因无关。

Effect of WT1 gene expression on cell growth and proliferation in myeloid leukemia cell lines

Objective: To explore the effect of WT1 antisense oligonucleotide(AS oligo) on cell proliferation and apoptosis in myeloid leukemia cell lines. Methods:K562 and HL 60 cells were cultivated with WT1 AS oligo. The cell proliferation, apoptosis,cell cycle and gene expression were examined by MTT colorimetry, FACS and RT PCR. Results: WT1 AS oligo could inhibit the proliferation of K562 cell and induce apoptosis of K562 and HL 60 cells.On the contrary, the growth of HL 60 cells and the expression of WT1, mdm2 and bcl 2 genes were unaffected. Conclusion: WT1 gene is related to the proliferation and apoptosis of leukemic cells. WT1 gene could suppress cell apoptosis independent of status of p53 and bcl 2 genes. It might play an role in leukemogenesis.