麝香保心丸对家兔血管球囊损伤后胶原增生的影响

目的　探讨血管球囊损伤后血清I型前胶原羧基端肽浓度和Ⅰ型胶原基因表达的变化及麝香保心丸对其影响。方法　将 4 5只实验兔随机分为 3组 ,每组 15只 :血管成形术组、血管成形术 +麝香保心丸组及对照组。应用球囊拉伤动脉内膜加高脂饲养的方法建立兔髂动脉硬化模型并行球囊扩张血管成形术 ,采用放射免疫法和核酸分子杂交技术对血清I型前胶原羧基端肽和髂动脉壁平滑肌细胞的Ⅰ型胶原基因的表达情况进行观察。结果　术后 1周时血管成形术组血清I型前胶原羧基端肽较对照组水平增加 (I型前胶原羧基端肽为 9.5 4± 1.4 9,P <0 .0 5 ) ,Ⅰ型胶原基因表达增加 ,经计算机图像辉度扫描定量分析结果为 (16 0± 12 ,P <0 .0 5 ) ,2、4周时显著增加 (P<0 .0 1) ,(血清I型前胶原羧基端肽为 14 .2 1± 2 .6 7和 15 .6 3± 6 .87,Ⅰ型胶原基因表达为 4 16± 16和 4 38± 17) ,血管成形术 +麝香保心丸组血清I型前胶原羧基端肽无显著增加、Ⅰ型胶原基因未表达。结论　血管内皮损伤可致Ⅰ型胶原合成增加 ,麝香保心丸对其有抑制作用

Effect of Musk Pill on Collagen Proliferation of Artery after Balloon Injury

Aim To study the changes of serum type I procollagen carboxyl terminal peptide (PIP) and the expression of type I collagen after balloon injury in rabbit and the effects of musk pill (MP) on it. Methods 45 rabbits were divided randomly into three groups: group of angioplasty (AP), group of AP plus musk pill (AP+MP) and group of control. A reproducible rabbit model of atherosclerosis was established with cholesterol feeding after iliac artery injury induced by an inflated balloon and balloon angioplasty was carried out. The expression of type I collagen gene in the smooth cells(SMC) of the iliac artery and serum PIP was respectively observed by molecular hybridization and radioimmunoassay. Results The level of serum PIP increased (9.54±1.49, p<0.05) in AP group compared with in control group. The expression of type I collagen(160±12,p<0.05) in AP group increased compared with in control group one week after the operation, and markedly increased at 2 and 4 weeks after the operation(p<0.01)(serum PIP: 14.21±2.67 and 15.63±6.87, the expression of type I collagen: 416±16 and 438±17), while no expression of type I collagen in AP+MP group. Conclusion These results suggest that iliac artery balloon injury may induce increase of type I collagen synthesis,which can be inhibited by MP.