聚乙烯醇-壳聚糖-胶原组织工程支架的研究

目的 以聚乙烯醇（PVA）、壳聚糖（Cs）和胶原（Col）为主要原料制备PVA-Cs—Col复合支架，并研究其作为组织工程支架材料的可行性。方法 把聚乙烯醇、壳聚糖和胶原按一定配比复合，测定复合材料的含水率、膨胀率和力学性能，扫描电镜观察材料横截面的组织形态。结果 不同分子量PVA与不同质量的cs和Col复合，得到的复合支架材料湿态抗张强度为5．70MPa，含水率在60．15％-72．50％，膨胀率在185．33％～317．57％。不同配比的复合支架具有不同的内部组织形态结构。结论 PVA—Cs—Col复合支架材料具有较高的含水率和适宜的膨胀率，内部孔洞丰富，Cs：PVA：Col的质量比为30：15：0．20时，复合支架综合性能较佳，适合用于组织工程支架材料。

Polyvinyl alcohol-chitosan-collagen as scaffolds for tissue engineering

Objective To fabricate a novel polyvinyl alcohol (PVA)-chitosan(Cs)-collagen (Col) composite material and to confirm the feasibility of its application as a scaffold in tissue engineering.Methods PVA was blended with chitosan and collagen.The water content,swelling ratio,and tensile strength of the scaffolds were tested.SEM was used to observe the histological modality of the cross section.Results Scaffolds which al'e composite with different molecular weight and different amount of Cs and Collagen ale made,with water content ranging from 60.15% to 72.50% and swelling ratio being from 185.33% to 317.57%.The tensile strength of the composite material is 5.70MPa.The inner modality and structure of the scaffolds varied as the proportion of the chemical components changed.Conclusion PVA-Cs-Col scaffolds have high water content and proper swelling ratio and ale rich in porous structure.When the blending proportion is Cs:PVA:COI=30:15:0.20,the scaffold performs best,which shows to be a suitable structure for tissue engineering scaffold.