宫颈癌患者外周血T淋巴细胞亚群及血VEGF水平的临床意义

目的探讨宫颈癌患者外周血T淋巴细胞亚群及血VEGF水平的临床意义。方法采用流式细胞仪(FCM)对34例宫颈癌患者外周血T淋巴细胞亚群进行检测,同时应用ELISA法检测血VEGF水平。随机抽取健康女性20例为对照组,比较两组T淋巴细胞亚群及VEGF。结果宫颈癌患者外周血CD4+、CD4+/CD8+比值较对照组均明显下降(P<0.05),而CD8+水平、IL-6、VEGF显著升高。IFN-γ水平与对照组无明显差异性。肿瘤期别越晚,CD4+/CD8+比值越低,CD8+、IL-6、VEGF水平越高,IFN-γ水平则无明显差异性。结论宫颈癌患者T淋巴细胞亚群改变导致细胞免疫功能降低,肿瘤细胞发生免疫逃逸,促进宫颈癌的发生、发展;VEGF诱导肿瘤血管形成,促进宫颈癌的侵袭、转移。

Clinical Significance of Peripheral Blood T Lymphocyte Subsets and Blood Levels of VEGF in Patients with Cervical Cancer

Objective To study the clinical significance of T-cells subgroup and vascular endothlial growth factor level in patients with cervical cancer.Methods The levels of T-cells subgroup and vascular endothelial growth factor（VEGF） were investigated in 34 patients with cervical cancer by flow cytometry（FCM） and enzyme-linked immunosorbent assay（ELISA）.20 healthy women were selected randomly as the control group.Results In group of cervical cancer,the blood CD4＋ level and the ratio of CD4＋/CD8＋ were obviously lower than that of control group,but the levels of CD8＋、IL-6、VEGF were significantly higher than in control group,which changed with cervical cancer classification.There was no significantly difference of the IFN-γ level between two groups.Conclusion The change of T-cells subgroup level in patients with cervical cancer may lead to a decreased cytoimmunity functions,the tumor immune escape caused the tumorigenesis and enhanced the progression of cancer;on the other hand,VEGF induced the angiogenesis of cancer,which promoted the metastasis of cervical cancer.