Characterization of cytosolic glutathione S-transferases in California Halibut (Paralichthys californicus) |
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| Authors: | Donham Rachel T Luna Andrea D Chang Sandra Morin Dexter Jewell William T Tjeerdema Ronald S |
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| Affiliation: | Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA. |
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| Abstract: | Two cytosolic glutathione S-transferase (GST) classes were isolated and characterized from California halibut (Paralichthys californicus) liver. Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as theta and alpha by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) was determined to be 0.23+/-0.003 U/mg cytosolic protein. |
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