Immunohistochemical Expression of p16, p53, and p63 in Colorectal Adenomas and Adenocarcinomas |
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Authors: | Fabiana Pirani Carneiro MD Leandra Náira Zambelli Ramalho MD PhD Sérgio Britto-Garcia MD PhD Alfredo Ribeiro-Silva MD PhD Sérgio Zucoloto MD PhD |
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Institution: | 1. Department of Pathology, Faculty of Medicine of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, S?o Paulo, Brazil 2. Department of Pathology, Faculty of Medicine of Ribeir?o Preto, University of S?o Paulo, Av Bandeirantes 3900, 14049-900, Ribeir?o Preto, S?o Paulo, Brazil
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Abstract: | Purpose The aim of this study was to investigate the immunohistochemical expression of p16, p53, and p63 proteins according to some
pathologic parameters related to colorectal adenomas and adenocarcinomas such as grade of dysplasia and histologic type.
Methods Immunohistochemistry with the antibodies p16, p53, and p63 was performed in tubular, tubular-villous, and villous adenomas
(n = 30) and in well, moderately, and poorly differentiated adenocarcinomas (n = 30). The p63-positive cases were submitted
to double immunolabeling with the cytokeratin 5 (CK5).
Results The p16 and p53 labelings were observed in some adenomas and adenocarcinomas but without any association with p63 expression,
histologic type, or grade of differentiation of the neoplasm. P63 expression was found mainly in the villous adenomas and
in the poorly differentiated adenocarcinomas. The poorly differentiated adenocarcinomas also exhibited coexpression of CK5
and p63.
Conclusions Despite both p16 and p53 having been detected in colorectal neoplasms, they were not related to the different histologic variables
nor to the expression of p63. However, p63 expression was closely associated with villous adenomas and poorly differentiated
adenocarcinomas. Thus, p63 may represent a marker of poor differentiation in colorectal neoplasms. The coexpression of p63
and CK5 observed in this study could be related to divergent differentiation during the development of colorectal cancer,
although further studies are warranted to refine the understanding of this process. |
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Keywords: | p16 p53 p63 Adenoma Adenocarcinoma |
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