猪肺表面活性物质对兔油酸性急性肺损伤肺力学及组织学的影响

目的探讨猪肺表面活性物质（ＰＳ）对家兔油酸性急性肺损伤（ＡＬＩ）肺力学及组织学的影响。方法２０只麻醉兔静注０．１ｍｌ／ｋｇ油酸致肺损伤后接受机械通气，治疗组（ＰＳ，ｎ＝１０）气管内注入５０ｍｇ／ｋｇ猪ＰＳ；对照组（ＣＯＮ，ｎ＝１０）气管内注入同体积ＮＳ，实验过程中观察肺动态顺应性（Ｃｄ）的变化，兔处死后取标本作光镜及电镜组织学观察。结果ＰＳ可明显增加损伤肺的Ｃｄ（Ｐ＜０．０５），病理组织学评分及电镜观察示ＰＳ组肺组织病理改变较ＣＯＮ组明显减轻。结论猪ＰＳ可改善油酸性肺损伤肺顺应性和肺的病理形态学变化，为ＰＳ治疗ＡＬＩ提供了理论依据。

THE EFFECTS OF PORCINE PULMONARY SURFACTANT ON THE LUNG MECHANICS AND HISTOLOGY OF RABBITS WITH OLEIC ACID-INDUCED LUNG INJURY

Objective The effects of porcine pulmonary surfactant (PS) instillation in rabbits on lung mechanics and histology with oleic acid-induced lung injury were investigated.Methods 20 anesthetized rabbits were received oleic acid intravenously at a dose of 0.1ml/kg. They were mechanically ventilated after lung injured and randomly allocated into two groups: Group PS intratracheal instilled PS of 50ml/kg; Group CON received normal saline (NS). Lung dynamic compliance(Cd) were measured for 4 h. Lungs pathologic histology were obtained after the animals were killed.Results Lung dynamic compliance were improved after PS replacement ( P <0.05). Lung tissue damages, being evaluated by pathologic scoring or transmission electron microscope, were significantly reduced by PS replacement.Conclusion It is suggested that the instillation of PS mitigates the degree of lung injury and has protective effects on the acute lung injury induced by oleic acid.