利多卡因调控微小RNA-195对骨肉瘤MG63细胞增殖、凋亡及化疗敏感性的影响

目的 探讨利多卡因(lidocaine)对骨肉瘤MG63细胞增殖、凋亡及化疗敏感性的影响及其可能的作用机制.方法 研究于2018年5―12月,体外培养骨肉瘤MG63细胞,不同浓度的利多卡因干预MG63细胞,采用甲基噻唑基四唑(MTT)法检测MG63细胞存活率及顺铂半抑制浓度(IC50值);流式细胞术检测细胞凋亡率;实时...

Effect of lidocaine of proliferation, apoptosis and chemosensitivity of osteosarcoma MG63 cells by regulating miR-195

Objective To investigate the effect of lidocaine on proliferation, apoptosis and chemosensitivity of osteosarcoma MG63 cells and its possible mechanism.Methods The study started May 2018 to December 2018, osteosarcoma MG63 cells were cultured in vitro, and MG63 cells were treated with different concentrations of lidocaine. MTT was used to detect the survival rate of MG63 cells and the semi-inhibitory concentration of cisplatin (IC50 value). Flow cytometry was used to detect the apoptosis rate. Real-time quanti-tative polymerase chain reaction (qRT-PCR) was used to detect the expression levels of microRNA-195 (miR-195) and multidrug resis-tance-associated protein 1 (MRP1). The proliferation, apoptosis and IC50 value of MG63 cells were observed after overexpression of miR-195 or inhibition of miR-195 expression. The proliferation and apoptosis-related protein expression were detected by Western blot.Results Lidocaine had a certain inhibitory effect on the proliferation of MG63 cells. Compared with the 0 mmol/L group, the apoptosis rate of lidocaine (18.35±1.85)%, (30.25±3.03)% vs. (8.22±0.82)%], Cleaved-caspase-3 (0.65±0.06), (1.00±0.10) vs. (0.15±0.02)] and the expression levels of miR-195 (1.86±0.18), (2.25±0.22) vs. (1.00±0.10)] in 2 mmol/L, 4 mmol/L groups were increased, MRP1 mRNA expression levels (0.72±0.08), (0.42±0.05) vs. (1.00±0.11)], cisplatin IC50 value (6.59±0.71) mg/L, (2.16±0.23) mg/L vs. (9.05±0.92) mg/L] were decreased, the difference was statistically significant (P<0.05), and miR-195 overexpression in MG63 cells had a simi-lar effect. Inhibition of miR-195 expression reversed the effect of lidocaine on proliferation, apoptosis and chemosensitivity of MG63 cells.Conclusion Lidocaine can inhibit the proliferation of osteosarcoma MG63 cells and induce apoptosis by regulating miR-195,and enhance its sensitivity to cisplatin chemotherapy.