两种GnRH类似物在体外受精-胚胎移植中对早期胚胎发育的影响

目的 比较体外受精-胚胎移植(IVF - ET)中促性腺激素释放激素激动剂(GnRH -a)长方案与促性腺激素释放激素拮抗剂(GnRH -A)方案早期胚胎发育的多项指标,以阐明促性腺激素释放激素(GnRH)类似物对早期胚胎发育的影响.方法 将100例卵巢储备正常的行IVF- ET助孕治疗患者随机分为GnRH -a组和G...

Influence of two GnRH analogues upon early embryo development in vitro fertilizationembryo transfer

Objective To compare the indicators of early embryo development of gonadotropin-releasing hormone antagonist ( GnRH - a ) long protocol with gonadotropin-releasing hormone agonist ( GnRH - A )protocol,to investigate the influence of GnRH analogues upon early embryo development in vitro fertilization - embryo transfer ( IVF-ET ).Methods 100 patients with normal ovarian reserve undergoing IVF-ET were classified into GnRHa group and GnRHA group randomly.Compared the laboratory results and the radio of blastomere number,vacuole,non-symmetry,multinuclear,and fraction of D2,D3 embryo; and compared the radio of transfer-embryo's cell number between pregnancy and nonpregnancy in fresh and frozen embryo transfer ( FET ) of two groups.Results The rates of normal fertility,normal cleavage,available embryo,and high-quality embryo were not significantly different between two groups(P ＞ 0.05).Otherwise,the radios of D2 multinuclear-embryo and D2,D3 fraction-embryo of Group 1 were significantly lower than Group 2; the radios of D2 ＞ 4-cells embryo and D3 8-cells embryo of Group 1 were significantly more than Group 2,which reached statistical difference(P ＜ 0.05).The pregnancy rate in fresh embryo transfer of Group 1 was higher than Group 2,which there was statistical differences between two groups(P＜ 0.05).No statistical differences were observed on the pregnancy rates in FET between two Groups (P＞ 0.05).There was no significant differences on implantation rates and the radio of cell number of transfer-embryo between pregnancy and non-pregnancy in fresh embryo transfer of Group 1 (P＞ 0.05).The number of 8-cells embryo in pregnancy was more than non-pregnancy in Group 2 ; there was significant difference(P＜ 0.05).Conclusion Early embryonic cleavage of GnRH-A protocol was slower than that of GnRH-A long protocol; and the radios of multinuclear and fraction embryo of GnRH-A protocol were higher than GnRH-A long protocol.8-cells embryo-transfer in fresh and frozen embryo transfer can contribute to increasing pregnancy rate in GnRH-A protocol.