疏经防痛胶囊的定性定量分析

目的:研究疏经防痛胶囊的质量控制方法。方法:采用TLC对疏经防痛胶囊中丹参、当归、乳香及没药进行定性鉴别;采用HPLC方法,以乙腈-0.1%磷酸溶液为流动相,进行梯度洗脱,在检测波长230 nm处同时测定芍药苷、柚皮苷、新橙皮苷及丹酚酸B共4种成分含量。结果:TLC鉴别分离度好,专属性强。芍药苷在25.30~506.00 mg·L~(~(-1)),柚皮苷在12.68~253.60 mg·L~(~(-1)),新橙皮苷在15.24~304.80 mg·L~(~(-1)),丹酚酸B在27.72~554.30 mg·L~(~(-1))分别具有良好的线性关系,相关系数分别为1.000 0,0.999 9,0.999 9,0.999 9,平均加样回收率分别为100.69%(RSD 0.9%),100.08%(RSD 1.6%),100.85%(RSD 1.2%)和100.42%(RSD 2.0%)。结论:该质量控制方法简便、准确、重复性好,可有效的控制疏经防痛胶囊质量。

Qualitative and Quantitative Analysis of Shujing Fangtong Capsule

Objective: To establish the method for quality control of Shujing Fangtong capsule. Method: TLC method was used to qualitatively identify Salvia Miltiorrhiza, Angelica Sinensis Radix, Olibanum and Myrrha in Shujing Fangtong capsule;the contents of paeoniflorin, naringin, neohesperidin and gsalvianolic acid B were simultaneously determined by HPLC, with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution at a detection wavelength of 230 nm. Result: The TLC identification method was distinct and highly specific. Paeoniflorin, naringin, neohesperidin and gsalvianolic acid B showed good linear relationship in ranges of 25.30-506.00 mg·L^-1 (r=1.000 0), 12.68-253.60 mg·L^-1(r=0.999 9), 15.24-304.80 mg·L^-1 (r=0.999 9) and 27.72-554.30 mg·L^-1(r=0.999 9) respectively. The average recovery rate of samples was 100.69%(RSD 0.9%), 100.08%(RSD 1.6%), 100.85%(RSD 1.2%) and 100.85%(RSD 2.0%) respectively. Conclusion:This quality control method is simple, accurate and reproducible, which can be used to effectively control the quality of Shujing Fangtong capsule.