| HPLC法测定北败酱中绿原酸含量 |
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| 引用本文： | 姜茁松,李井涛. HPLC法测定北败酱中绿原酸含量[J]. 长春中医药大学学报, 2016, 32(2): 259-260. DOI: 10.13463/j.cnki.cczyy.2016.02.013 |
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| 作者姓名： | 姜茁松 李井涛 |
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| 作者单位： | 吉林省药品检验所,长春,130033;吉林省药品检验所,长春,130033 |
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| 摘 要： | 目的：建立HPLC法测定北败酱中绿原酸含量的方法。方法采用色谱柱Diamonsil（5μm，4.6 mm ×250 mm）；流动相乙腈-0.4％磷酸溶液（13：87）；检测波长327 nm；流速1.0 mL/min；柱温35℃。结果绿原酸在0.04~0.36μg（ r＝0.9995）范围内，线性关系良好，加样回收率（ n＝6）为99.27％（ RSD＝1.03％）。结论本法简便，准确，重现性好，可作为评价北败酱的质控方法。
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| 关 键 词： | 北败酱 绿原酸 高效液相色谱 |
Determination of chlorogenic acid in ixeris chinensis by HPLC |
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| JIANG Zhuosong,LI Jingtao. Determination of chlorogenic acid in ixeris chinensis by HPLC[J]. Journal of Changchun College of Traditional Chinese Medicine, 2016, 32(2): 259-260. DOI: 10.13463/j.cnki.cczyy.2016.02.013 |
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| Authors: | JIANG Zhuosong LI Jingtao |
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| Abstract: | Objective To establish a method for determination of chlorogenic acid in Ixeris Chinensis by HPLC. Methods A column of Diamonsil(5μm,4. 6 mm × 250 mm)was adopted,and the mobile phase was the mixture of acetonitrile-0. 4% phosphoric acd and water(13:87)at a flow rate of 1. 0 mL/min;The wavelength was set at 327nm. Results There was a good linear relationship in the concentration range of 0 . 04~0 . 36 μg( r=0 . 999 5 )of chloro-genic acid;The average recovery(n=6)was 99. 27%(RSD=1. 03%). Conclusion The method is simple,accu-rate,and can be used for the quantitative analysis of Ixeris Chinensis. |
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| Keywords: | ixeris chinensis chlorogenic acid HPLC DAD |
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