复方丹酚滴丸中冰片对丹酚酸大鼠在体肠吸收特性的影响

目的:研究复方丹酚滴丸中不同质量浓度冰片配伍丹酚酸后对丹酚酸B,紫草酸,迷迭香酸3个成分在小肠的吸收速率常数(Ka)和表观吸收系数(P_(app))的影响。方法:采用大鼠在体单向肠灌注试验考察吸收过程,以HPLC测定丹酚酸B,紫草酸和迷迭香酸的含量,流动相1%冰乙酸水溶液(A)~(~(-1))%冰乙酸甲醇溶液(B)梯度洗脱(0~10 min,10%~20%B;10~40min,20%~47%B;40~50 min,47%~70%B),检测波长286 nm,流速0.8 m L·min~(~(-1))。结果:高、中、低质量浓度冰片配伍丹酚酸后,Ka和Papp大多有所增加,其中以100 mg·L~(~(-1))冰片组的促吸收效果最优,该组丹酚酸B,紫草酸,迷迭香酸的全肠段Ka分别为不加冰片的2.31,2.33,3.38倍。结论:不同浓度冰片配伍丹酚酸后,对丹酚酸B,紫草酸和迷迭香酸在大鼠各肠段的吸收具有一定促进作用,浓度对促吸收效果有一定影响;增加冰片的浓度,促吸收作用有所提高,但当冰片到一定浓度后,促吸收作用有所减弱。

Effects of Borneolum Syntheticum on Intestinal Absorption of Salvianolic Acids in Compound Danfen Dropping Pills

Objective: To investigate effects of salvianolic acids compatible with borneol at different concentration on absorption rate constant (K_a) and apparent absorption coefficient (P_app) of salvianolic acid B, lithospermic acid and rosmarinic acid in rat small intestine. Method: An in situ intestinal perfusion model was employed to investigate absorption of salvianolic acids in rat small intestine. HPLC was used to determine contents of salvianolic acid B, lithospermic acid and rosmarinic acid, mobile phase was 1% glacial acetic acid water solution-1% glacial acetic acid methanol solution, detection wavelength was 286 nm, flow rate was 0.8 mL·min^-1. Result: Compatibility of salvianolic acids with different concentration of borneol, both K_a and P_app were increased, enhancing effect of borneol with the concentration of 100 mg·L^-1 was optimum. K_a of salvianolic acid B, lithospermic acid and rosmarinic acid in borneol with the concentration of 100 mg·L^-1 was 2.31, 2.33, 3.38 times as long as the blank group. Conclusion: After compatibility of salvianolic acids with different concentrations of borneol, there is significant promoting effect of salvianolic acid B, lithospermic acid and rosmarinic acid in absorption of all segments of intestine. The capacity of enhancing effect is influenced by the concentration of borneol. When the concentration of borneol increases, the enhancing effect of salvianolic acids in intestinal absorption increases, but it significantly decreases after the concentration of borneol increases to some degree.