| UPLC-MS/MS快速测定茯苓中去氢土莫酸和茯苓酸的含量 |
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| 引用本文： | 李健康,张敏,刘校妃,唐怡,陈洋,严志宏,袁金斌. UPLC-MS/MS快速测定茯苓中去氢土莫酸和茯苓酸的含量[J]. 中国实验方剂学杂志, 2017, 23(4): 85-88 |
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| 作者姓名： | 李健康 张敏 刘校妃 唐怡 陈洋 严志宏 袁金斌 |
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| 作者单位： | 江西中医药大学 现代中药制剂教育部重点实验室, 南昌 330004,南昌市食品药品检验所, 南昌 330038,江西中医药大学 现代中药制剂教育部重点实验室, 南昌 330004,江西中医药大学 现代中药制剂教育部重点实验室, 南昌 330004,江西中医药大学 现代中药制剂教育部重点实验室, 南昌 330004,江西中医药大学 现代中药制剂教育部重点实验室, 南昌 330004,江西中医药大学 现代中药制剂教育部重点实验室, 南昌 330004 |
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| 基金项目： | 国家自然科学基金项目（81260605，81560648） |
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| 摘 要： | 目的:建立一种测定茯苓药材中茯苓酸和去氢土莫酸含量的UPLC-MS/MS方法。方法:采用ZORBAX Eclipse plus C18色谱柱(2.1 mm×100 mm,1.8μm),流动相乙腈-1.0 mmol·L-1乙酸铵(80∶20),流速0.3 m L·min-1,柱温40℃,进样量5.0μL。ESI离子源,负离子模式,多反应监测模式(MRM),检测离子对分别为茯苓酸m/z 527.40/465.30,去氢土莫酸m/z 483.20/421.20。结果:茯苓酸和去氢土莫酸分别在26.2~524μg·L-1(r=0.999 8)和24.2~488μg·L-1(r=0.999 9)线性良好,分析时间为6 min,平均回收率分别为101.4%(RSD 1.2%)和99.8%(RSD 1.2%)。结论:该方法准确、快速、可靠、灵敏,可作为茯苓酸和去氢土莫酸的一种快速定量分析方法。
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| 关 键 词： | 超高效液相色谱-质谱联用 茯苓 茯苓酸 去氢土莫酸 |
| 收稿时间： | 2016-03-05 |
Rapid Determination of Dehydrotumulosic Acid and Pachymic Acid in Poria by UPLC-MS/MS |
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| LI Jian-kang,ZHANG Min,LIU Xiao-fei,TANG Yi,CHEN Yang,YAN Zhi-hong and YUAN Jin-bin. Rapid Determination of Dehydrotumulosic Acid and Pachymic Acid in Poria by UPLC-MS/MS[J]. China Journal of Experimental Traditional Medical Formulae, 2017, 23(4): 85-88 |
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| Authors: | LI Jian-kang ZHANG Min LIU Xiao-fei TANG Yi CHEN Yang YAN Zhi-hong YUAN Jin-bin |
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| Affiliation: | Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM), Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China,Nanchang Institute for Food and Drug Control, Nanchang 330038, China,Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM), Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China,Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM), Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China,Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM), Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China,Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM), Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China and Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM), Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China |
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| Abstract: | |
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| Keywords: | UPLC-MS/MS Poria polyporenic acid dehydrotumulosic acid |
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