基质金属蛋白酶对激素治疗特发性肺间质纤维化效果的评价作用

目的探讨基质金属蛋白酶(MMPs)的表达与激素治疗特发性肺间质纤维化(IPF)效果的关系。方法应用明胶酶谱法检测37例IPF进展期患者分为糖皮质激素治疗前组(IPF1组)、治疗3个月组(IPF2组)和治疗6个月组(IPF3组)和35例健康者(对照组)血清中以酶原形式表达的明胶酶A(ProMMP-2)和B(ProMMP-9)及以活性形式表达的明胶酶A(MMP-2)和B(MMP-9)的水平。结果IPF1组血清ProMMP-9、MMP-9、ProMMP-2和MMP-2的水平与对照组比较,差异有统计学意义(P<0.05);IPF2和IPF3组患者血清ProMMP-9、MMP-9、ProMMP-2和MMP-2水平与IPF1组比较,差异亦有统计学意义(P<0.05),但与对照组比较,差异无统计学意义(P>0.05)。结论MMPs参与了肺损伤、修复及肺间质纤维化的形成过程,其表达水平与进展期肺纤维化的程度及激素治疗效果相平行。

Expression of Matrix Metalloproteinases:A Study of Its Evaluation of Glucocorticoid Effect on Idiopathic Pulmonary Fibrosis

Objective To investigate the relationship between expression of matrix metalloproteinases (MMPs) and effect of glucocorticoid in treatment of idiopathic pulmonary fibrosis (IPF).Methods Totally 37 IPF patients were divided into three groups:IPF 1 group (without administration of glucocorticoid),IPF 2 group (administration of glucocorticoid for 3 months) and IPF 3 group (administration of glucocorticoid for 6 months),and 35 healthy subjects were enrolled as the control.Gelatin Zymography was used to detect the expressions of serum progelationase A (proMMP-2) and B (proMMP-9),gelationase A (MMP-2) and B (MMP-9).Results The expressions of serum ProMMP-9,MMP-9,ProMMP-2 and MMP-2 in IPF1 group were much higher than those in the control group(P<0.05),and also higher than those in IPF 2 and IPF 3 groups(P>0.05).However,IPF2 group and IPF3 group were not significantly different from the control group in the expressions of serum ProMMP-9,MMP-9,ProMMP-2 and MMP-2(P>0.05).Conclusion MMPs may be associated with the process of lung damnification and restoration and the development of IPF,and the expressive level is parallel to the degree of IPF at progressive stage and the therapeutic effect of glucocorticoid.