氢化可的松诱导的大鼠记忆减退与氧自由基的关系

目的　研究氢化可的松（HC）对大鼠学习记忆功能的影响及与氧自由基的关系。方法　迷宫法检测实验大鼠的学习记忆功能；Lowry法及硫代巴比妥酸法分别测定胞浆及线粒体的蛋白质及MDA含量；分光光度法及荧光分光光度法分别测定胞浆及线粒体的GSH及GSSG的含量；黄嘌呤氧化酶法及紫外分光光度法分别测定胞浆及线粒体的Mn-SOD及CAT的含量。结果　HC可引起老龄前期大鼠记忆障碍，同时使脑细胞浆与线粒体产生MDA及GSSG增加、GSH及GSH/GSSG比值下降以及Mn-SOD及CAT活性降低。结论　HC同步抑制老龄前期大鼠学习记忆功能是与其降低机体的抗氧化能力和增加氧自由基的损伤有关。

HYDROCORTISONE INDUCED DECREASE OF LEARNING AND MEMORY FUNCTIONS IN RATS AND ITS RELATED TO OXYGEN FREE RADICAL

AIM　To study the effects of hydrocortisone（HC）on the learning and memory function and it′s mechanism related to the oxygen free radical in presenium rats. METHODS　The learning and memory function was determinated by Y-Maze; The contents of protein and MDA in cytoplasm and mitochondria were assayed by methods of Lowry and TBA respectively; The contents of GSH, GSSG, Mn-SOD and CAT in cytoplasm and mitochondria were assayed by spectroscopy,fluorescence spectroscopy, XO and UV spectroscopy respectively. RESULTS　The learning and memory function in presenium rats treated with HC（10 mg。kg^-1。d^-1×21 d, sc） decreased significantly, accompanied with increased levels of MDA and GSSG, and lowered levels of GSH, SOD and CAT. CONCLUSION　HC can induce the change of learning and memory via production of oxygen free radical.