| 壳聚糖聚乳酸膜的制备和毒性试验研究 |
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| 引用本文： | 陈康,李瑛,谢良宪. 壳聚糖聚乳酸膜的制备和毒性试验研究[J]. 西南国防医药, 2011, 21(6): 585-587. DOI: 10.3969/j.issn.1004-0188.2011.06.002 |
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| 作者姓名： | 陈康 李瑛 谢良宪 |
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| 作者单位： | 解放军324医院口腔科,重庆,400020 |
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| 基金项目： | 成都军区"十一五"指令性课题 |
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| 摘 要： | 目的研制结肠瘘的一期修复生物膜,并进行相关急性毒性和细胞毒性试验。方法制备聚乳酸膜和壳聚糖聚乳酸膜,按照药典要求进行壳聚糖聚乳酸膜的小鼠急性全身毒性试验和细胞毒性试验（台盼蓝计数细胞活性和MTT法检测细胞活性）。结果建立了壳聚糖聚乳酸膜的制备方法,聚乳酸膜与壳聚糖聚乳酸膜的物理性能比较无统计学差异;小鼠急性全身毒性试验无动物死亡和异常反应;细胞毒性试验显示,两膜比较无统计学差异。结论壳聚糖聚乳酸膜的物理指标达到药典要求,无急性全身毒性反应和细胞毒性反应,初步评价生物相容性好。
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| 关 键 词： | 聚乳酸 壳聚糖 壳聚糖聚乳酸膜 毒性 |
Preparation and toxicity test of chitosan-polylactic acid film |
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| Chen Kang,Li Ying,Xie Liangxian. Preparation and toxicity test of chitosan-polylactic acid film[J]. Medical Journal of National Defending forces in Southwest China, 2011, 21(6): 585-587. DOI: 10.3969/j.issn.1004-0188.2011.06.002 |
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| Authors: | Chen Kang Li Ying Xie Liangxian |
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| Affiliation: | Chen Kang,Li Ying,Xie Liangxian Department of Stomatology,Hospital 324 of PLA,Chongqing,400020,China |
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| Abstract: | Objective To manufacture a biomembrane for the primary repair of colonic fistula,and to evaluate its acute general toxicity and cytotoxicity.Methods After the preparation of polylactic acid film(PAF)and chitosan-polylactic acid film(CPAF),acute general toxicity and cytotoxicity examinations were performed with trypanblau cytoactive count and MTT reduction assay according to pharmacopeia for CPAF.Results A method for the preparation of CPAF was successfully established.There were no significant differences i... |
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| Keywords: | polylactic acid chitosan chitosan-polylactic acid film toxicity |
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