7型腺病毒疫苗株87mu-97.4mu片段核苷酸序列分析

目的获得７型腺病毒（Ａｄ７）疫苗株８７ｍｕ－９７．４ｍｕ核苷酸序列，分析该区段的基因结构和功能。方法应用Ｓａｎｇｅｒ双脱氧法进行核苷酸序列分析。结果Ａｄ７疫苗株８７ｍｕ－９７．４ｍｕ全长３６９８个核苷酸，推测编码纤维蛋白（３２５个氨基酸）和Ｅ３区１５．４ｋＤ蛋白，Ｅ４区５个蛋白（ＯＲＦ１４．２，ＯＲＦ１５．７，ＯＲＦ８．１，ＯＲＦ４２．８和ＯＲＦ１０．３）。结论这一结果为阐明Ａｄ７的基因结构与功能及利用该病毒做为基因工程疫苗载体打下一定的基础

Sequence analysis of Ad7 vaccine strain 87mu97.4mu fragment

Adenovirus 7 vaccine strain(Ad7v) possesses potentiality to be used as a vector for producing recombinant vaccine. In order to construct Ad7 vector, the nucleotide sequence of a 3698 bp fragment(87 to 97.4 mu) of the virus DNA was analysed. The fragment encodes three ORFs namely, one ORF of early region 4(E4), one ORF of early region 3 and one ORF of fiber protein. The predicted amino acids of three ORFs(ORF 8.1 kD, 42.8 kD and 10.3 kD) share 33%, 43% and 44% homology with Ad2 E4 corresponding regions (ORF 3, ORF 4 and ORF 6/7) respectively. A comparison of the nucleotide and amino acid sequences of those fiber ORF demonstrated a higher homology between Ad7v and prototype strains of Ad7v than those between Ad7v and Ad7h. The Ad7v E3 15.4 kD protein shares 86%, 88%, 90% and 98.5% amino acid homology with the equivalent region of ad7h E3 15.3 kD protein, Ad4 E3 region 15 kD protein, Ad3 E3 15.3 kD and Ad7p E3 15.3 kD protein protein respectively, but only 68% with Ad2 E3 14.7 kD protein.