无症状不育病人精液抗沙眼衣原体IgG、IgM类别抗体的检测

目的 :探讨无症状不育病人精液抗沙眼衣原体 (Chlamydiatrachomatis,CT) IgG、 IgM抗体检测的临床意义。　方法 :随机选择 116例无生殖道感染症状的不育病人及 18例生育男性 ,作精液常规参数分析 ,然后分离精浆 ,测定其抗CT IgG、 IgM抗体。 　结果 :①不育病人精浆抗CT IgG、 IgM抗体阳性率分别为13 .8% ( 16/ 116)和3 .4% ( 4/ 116) ,而生育男性分别为 11.1% ( 2 / 18)和 0 ,两组相比差异均无显著性 (P均 >0 .0 5 )。② 116例不育病人中有 2 2例为无精子症。其余 94例病人中 ,精子密度异常组的精浆抗CT IgG、 IgM抗体阳性率分别为2 1.4% ( 6/2 8)和 7.1% ( 2 / 2 8) ,精子密度正常组则较低 ,分别为 12 .1% ( 8/ 66)和 3 .0 % ( 2 / 66) ,但差异均无显著性 (P均 >0 .0 5 ) ;精子活率正常组和异常组之间的精浆抗CT IgG、 IgM抗体阳性率也无明显差异 (P均 >0 .0 5 )。③ 116例不育病人中 3 0例CT阳性 ( 2 5 .9% )。　结论 :无症状不育病人的精浆抗CT IgG、 IgM抗体阳性率与生育男性相似 ,与其精液参数改变无关 ,不能作为CT感染的辅助指标

Detection of IgG and IgM Antibodies Against Chlamydia Trachomatis in Semen of Asymptomatic Infertile Patients

Objectives: To evaluate the clinical significance of the detection of IgG and IgM antibodies against Chlamydia trachomatis(CT) in semen of asymptomatic infertile patients. Methods: One hundred and sixteen asymptomatic infertile patients and eighteen fertile males were selected randomly. The routine parameter analysis of semen was fulfilled by computer aided semen analysis(CASA). Then the seminal plasma was separated and the IgG, IgM antibodies against CT in seminal plasma were determined with ELISA method. Results: IgG and IgM antibodies against CT were present in 13.8%(16/116) and 3.4%(4/116) of the semen of infertile patients, while for the fertile males the percentages were 11.1%(2/18) and 0, respectively. There were no differences between the two groups(P> 0.05). In the infertile patients, 22 patients were azoospermia. And in the rest 94 infertile patients, the percentages of IgG and IgM antibodies in abnormal sperm density group were 21.4%(6/28) and 7.1%(2/28), which were higher than those in normal group, but there were no statistical differences(P> 0.05). Similarly, the IgG, IgM antibodies were not correlated with the sperm motility(P> 0.05).The positive percentage of CT in 116 patients was 25.9%(30/116). Conclusions: The percentages of IgG and IgM antibodies against CT in semen of asymptomatic infertile patients are similar to that in fertile males, which do not correlate with the changes of semen parameters, and may not be used for indication of CT infection. Natl J Androl,2003,9(3):197-199