| 海南胆木药材指纹图谱研究 |
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| 引用本文： | 李海龙,谭银丰,赖伟永,刘明生,符乃光,张俊清.海南胆木药材指纹图谱研究[J].时珍国医国药,2009,20(11). |
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| 作者姓名： | 李海龙 谭银丰 赖伟永 刘明生 符乃光 张俊清 |
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| 作者单位： | 海南医学院·海南省热带药用植物研究开发重点实验室,海南,海口,571101 |
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| 基金项目： | 国家科技支撑计划项目课题，海南省海口市重点科技计划项目 |
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| 摘 要： | 目的 采用高效液相色谱法建立海南胆木药材的指纹图谱,为海南胆木药材的生产提供质量控制方法 .方法 采用高效液相色谱法, 色谱柱为Diamonsil TM C18(250 mm×4.6 mm,5 μm);检测波长为227nm;流动相为甲醇-0.10%磷酸水溶液,二元梯度洗脱.结果 海南胆木药材指纹图谱中,共有峰相对保留时间与相对峰面积的精密度、重复性和稳定性的RSD均小于3%,符合有关规定.结论 所建立指纹图谱可用于海南胆木药材的质量控制.
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| 关 键 词： | 海南胆木 高效液相色谱法 指纹图谱 质量控制 |
Studies on the Fingerprint Chromatography of Hainan Nauclea officinalis |
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| LI Hai-long,TAN Yin-feng,LAI Wei-yong,FU Nai-guang,LIU Ming-sheng,ZHANG Jun-qing.Studies on the Fingerprint Chromatography of Hainan Nauclea officinalis[J].Lishizhen Medicine and Materia Medica Research,2009,20(11). |
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| Authors: | LI Hai-long TAN Yin-feng LAI Wei-yong FU Nai-guang LIU Ming-sheng ZHANG Jun-qing |
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| Abstract: | Objective To establish the Fingerprint Chromatography of Hainan Nauclea officinalis by HPLC and provide the method for quality control of Hainan Nauclea officinalis. Methods An HPLC method with the Diamonsil TMC_(18) column(250 mm×4.6 mm,5μm) and a mixture of methanol-0.10% phosphoric acid solution(dual gradient elution) as the mobile phase was used. The dectection wavelength was set at 227nm. Results HPLC fingerprint chromatography of 10 batches of samples was established. The results showed that this method had a good repeatability and the 8 common peaks had an exclusive properties. Conclusion The fingerprint established can be used for quality control of Hainan Naucleae officinalis. |
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| Keywords: | Hainan Naucleae officinalis HPLC Fingerprint Quality control |
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