电针腧穴“胃病方”对急性胃黏膜损伤模型大鼠延髓和下丘脑c-fos表达的影响

目的:观察腧穴"胃病方"的针刺信号在急性胃黏膜损伤模型大鼠延髓和下丘脑中的传导途径。方法:将40只清洁级Wistar大鼠随机分为空白组、假模型组、模型组、胃病方组4组,每组10只。造模采用无水乙醇灌胃法。胃病方组取内关、中脘和足三里,针刺入穴位后施以疏密波,频率10 Hz～30 Hz,电流强度以大鼠肢体微颤为宜。电针20 min,电针治疗结束1 h后,以免疫组织化学检测延髓和下丘脑的c-fos表达。结果:与模型组比较,经电针胃病方治疗后,延髓和下丘脑中c-fos阳性细胞个数明显增多。结论:腧穴"胃病方"在针灸刺激后,c-fos阳性细胞在延髓的孤束核、三叉神经背核及背侧网状亚核等核团和下丘脑背外侧区表达较多。

Effects of 'Weibingfang' Stimulated by Electroacupuncture on Expression of c-fos Protein in Medulla Oblongata and Hypothalamus of Rats with Acute Gastric Mucosal Damage

Objective：To observe the signal transduction pathway of atacupoints of ＇Weibingfang＇stimulated by electroacupuncture（EA）in medulla oblongata and Hypothalamus of rats with acute gastric mucosal damage. Methods：40 Wistar rats （sanitary degree）were randomly divided into 4 groups （blank control group,model group,sham-model group, Weibingfang group,n=10）. Acute gastric mucosal damage models were induced by anhydrous alcohol-induced methods. EA （dilatational wave, 10 Hz-30 Hz,suitable current intensity）was applied to Weibingfang group. After treatment by EA for 1 h,c-fos protein in medulla and hypothalamus was detected by immunohistochemistry method. Result：Compared with model group, expression of c-los in medulla and hypothalamus of the rats was obviously increased in ＂Weibingfang＂group. Conclusion：Atacupoints of ＂Weibingfang＂stimulated by electroacupuncture can upregulate expression of c-los protein in nucleus tractus solitaries,trigeminal nuclei and subnucleus reticularis dorsalis of medulla and in dosal-lateral hypothalamus in rats with acute gastric mucosal damage.