丙酮酸乙酯对小鼠缺血再灌注肾脏细胞凋亡的影响

目的研究丙酮酸乙酯（EP）对小鼠缺血再灌注（I／R）肾脏组织凋亡相关基因表达及细胞凋亡的影响。方法8周龄雄性BABL／c小鼠52只，随机分为假手术组（n=10）、I／R组（建立I／R模型，n=10）、EP处理组（n=32）；EP处理组再分为EP预处理组（建模前30min给药）和I／R后4、6、12hEP处理组（分别于建模后4、6、12h给药），每组8只动物。TUNEL染色分析和比较各组凋亡细胞数量的变化；Real—timePCR检测肾脏组织凋亡相关基因Bcl-2、Bax、Caspas-3mRNA表达。结果TUNEL染色分析显示，与假手术组比较，I／R组凋亡细胞数量明显增多（P〈0．05）；EP预处理组凋亡细胞数量明显少于I／R组（P〈0．05）。Real-timePCR检测结果显示，与假手术组相比，I／R组Bax和Caspase-3mRNA表达显著升高，Bcl-2mRNA表达明显降低（P〈0．05）；与I／R组比较，EP预处理组和I／R后6hEP处理组的Bcl-2mRNA表达显著升高（P〈0．05），EP预处理组和I／R后各时间点EP处理组的Bax和Caspase-3mRNA表达明显降低（P〈0．05）。结论EP能有效抑制BABL／c小鼠肾脏I／R过程中的细胞凋亡，可能与其调控肾脏组织凋亡相关基因的表达有关。

Effects of ethyl pyruvate on apoptosis of renal cells with ischemia-reperfusion in mice

Objective To investigate the effects of ethyl pyruvate (EP) on expression of apoptosis related gene of renal tissues and apoptosis of renal cells with ischemia-reperfusion (I/R) in mice. Methods Fifty-two male BABL/c mice of 8 weeks old were randomly divided into sham operation group (n=10), I/R group (I/R model was established, n=10) and EP treatment group (n=32). EP treatment group was subdivided into EP pretreatment group (administration of EP 30 min before model establishment, n=8) and 4 h, 6 h and 12 h post-I/R EP treatment groups (administration of EP 4 h, 6 h and 12 h after model establishment, respectively, n=8 in each group). Cell apoptosis of each group was analysed by TUNEL staining, and the expression of apoptosis related gene Bcl-2, Bax and Caspase-3 mRNA was detected by Real-time PCR. Results TUNEL staining analysis indicated that the number of apoptotic cells in I/R group was much larger than that in sham operation group (P<0.05), and the number of apoptotic cells in EP pretreatment group was much smaller than that in I/R group (P<0.05). Real-time PCR revealed that the expression of Bax and Caspase-3 mRNA was significantly higher and the expression of Bcl-2 mRNA was significantly lower in I/R group than that in sham operation group (P<0.05), the expression of Bcl-2 mRNA was significantly higher in EP pretreatment group and 6 h post-I/R EP treatment group than that in I/R group (P<0.05), and the expression of Bax and Caspase-3 mRNA was significantly lower in EP pretreatment group and 4 h, 6 h and 12 h post-I/R EP treatment groups than that in I/R group (P<0.05). Conclusion EP can effectively inhibit apoptosis of renal cells with I/R in BABL/c mice, which may be related to the regulation of apoptosis related gene of renal tissues.