川芎嗪对胎鼠宫内生长受限的防治作用研究

目的观察川芎嗪对胎鼠子宫内生长受限(FGR)的防治作用,以及对胎鼠大脑的保护作用及其机制。方法将80只受孕SD大鼠随机分为对照组,模型组和川芎嗪高、低剂量组,每组20只,采用被动吸烟法在模型组和川芎嗪高、低剂量组大鼠体内建立胎鼠FGR模型。川芎嗪高、低剂量组大鼠妊娠第7~20天分别给予川芎嗪0.4和0.2g.kg-1灌胃,对照组和模型组给予等量0.9%氯化钠溶液灌胃。均每天1次。于受孕第21天取各组大鼠10只,剖宫取胎鼠,测定胎鼠体重、身长和脑立体定位图谱,并常规制作超薄切片。另10只待自然分娩后第1天制作标本,方法同前。结果与模型组比较,川芎嗪高、低剂量组胎鼠体重、身长均明显增加,且均差异有极显著性(均P<0.01)。电镜观察可见模型组幼鼠海马CA1区神经细胞线粒体嵴断裂、消失,染色质聚集、附着在核膜周围;川芎嗪高、低剂量组与对照组相似,仅部分神经细胞线粒体肿胀。结论川芎嗪可促进幼鼠生长发育,有效防治胎鼠FGR,并可保护幼鼠大脑发育。

Preventive and Therapeutic Effects of Tetramethylpyrazine on Rat Embryos with Fetal Growth Restriction

Objective To survey the preventive and therapeutic effects of tetramethylpyrazine(TMP) on rat embryos with fetal growth restriction(FGR) as well as its protective effects on the fetal brain. Methods 80 pregnant SD rats were randomly divided into 4 equal groups: the control group,the model group,high and low dose TMP groups.The FGR model was set up with the passive smoking method in each animal of the model,high and low dose TMP groups.From the 7~(th) to the 20~(th) gestation days,rats of the high and low dose TMP groups were given each 0.4 g and 0.2 g of TMP administered by gastrogavage q.d.,respectively.Animals of the control group and model group were given each equivalent amounts of 0.9% sodium chloride solution administered by gastrogavage q.d.as well.On day 21 of gestation, 10 rats from each of the 4 groups were sacrificed and the fetuses were taken for the measuring of body weight and body length as well as preparation of stereotaxic atlas of the brain and ultrathin brain sections for electron microscopic examination.The neonates given birth by the 10 remaining rats of each of the 4 groups were subjected to the same measurement and preparation as described above 1 day after the natural delivery. Results The fetuses of the rats of high and low dose TMP groups had significantly greater body weight and body length as compared with those of rats of the model gsoup(P<0.01).Disruption and even disappearance of mitochondrial cristae,chromatin aggregation and attachment around the nuclear membrane in neurous of the hippocampal CA1 region in neonates of the model group were demonstrated by electron microscopy.In contrast,only mitochondrial swelling was shown in a portion of the neurons of neonates of the high dose and low dose TMP groups,a condition similar to that in neonates of the control group. Conclusion Tetramethylpyrazine was shown to promote the growth and development of the rat fetus,effectively preventing and treating FGR as well as protecting the brain development of rat neonates.