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The development and application of solid-phase ELISA for the identification of avian influenza virus and its subtypes H5 and N3 in different biological samples
Authors:Matveeva V M  Koshemetov Zh K  Mamadaliev S M
Abstract:The paper presents the results of research on the development of effective solid-phase ELISA methods for the identification of avian influenza virus (AIV) and typing its H5 and N3 subtypes in different biological samples. Optimized schemes for the production of goat antisera to matrix protein, hemagglutinin H5 and neuraminidase N3 of AIV were proposed. They were used to produce active and specific immunoglobulins and conjugates. Conditions of performing solid-phase ELISA with use of these preparations were optimized.
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