卡介菌多糖核酸对结核性胸膜炎淋巴细胞表面粘附分子表达的影响

目的 探讨卡介菌多糖核酸（斯奇康）对结核性胸膜炎的免疫调节作用机制。方法用双抗体夹心酶联免疫吸附试验（ELISA）和间接酶标记的链酶卵白素—生物素检测法，观察卡介菌多糖核酸治疗前后结核性胸膜炎患者胸腔积液中可溶性细胞间粘附分子-1（sICAM-1）和外周血淋巴细胞上粘附分子配体CD11a，CD11b阳性表达百分率。结果 经卡介菌多糖核酸治疗后，患者胸腔积液中sICAM-1及外周血CD11a,CD11b表达显著降低（P〈0.001），且10例患者胸腔积液完全吸收。结论 卡介菌多糖核酸对结核性胸膜炎的免疫调节可能通过影响淋巴细胞上粘附分子的表达及细胞间粘附连接实现，以此抑制结核性胸膜炎的细胞免疫反应，减少胸腔积液渗出，控制患者病情。

Effect of BCG - PSN on Expression of Adhesion Molecule of Lymphocyte in Tuberculous Pleurisy

Objective To investigate the immunological mechanism of BCG-PSN ameliorating tuberculous pleurisy.Methods ELISA and avidin-biotin complex method were used to observe the positive expression percentage of adhere molecule CD11a and CD11b of lymphocyte in tuberculous pleurisy without and after using BCG-PSN,and compare the expression of sICAM-1 in tuberculous pleural fluid before and after using BCG-PSN.Results The levels of sICAM-1 in tuberculous pleural fluid and the expressive levels of adhere molecule CD11a and CD11b on the peripheral blood lymphocytes were lower significantly after using BCG-PSN(P < 0.001),and tuberculosis pleural fluid was absorbed absolutely in 10 patients.Conclusion BCG-PSN maybe immunoregulate tuberculous pleurisy by affecting the expression of adhere molecules and conjunctions of intercell,which restrains cell immune and is beneficial to ameliorate the tuberculous state of an illness and reduces pleural effusion.