血府逐瘀汤对动脉血栓模型大鼠抗凝系统的影响

目的 研究血府逐瘀汤不同剂量组对三氯化铁(FeCL3)所致大鼠颈总动脉血栓形成后抗凝血酶-Ⅲ(AT-Ⅲ) 和蛋白C (PC) 含量的影响.方法 选择SD大鼠60只,雌雄各半,随机分为空白组,模型组,阿司匹林组,血府逐瘀汤高剂量组、中剂量组、低剂量组,连续灌胃给药14 d,第14天除空白组外,均采用FeCL3外敷颈总动脉的方法复制动脉血栓模型,分别称量其血栓质量和测定血浆AT-Ⅲ和PC含量. 结果 模型组有明显的血栓形成,说明造模成功;与模型组比较,血府逐瘀汤高、中、低剂量组,阿司匹林组血栓质量减轻,其中中剂量组血栓质量最轻,差异有统计学意义(P<0.01);血府逐瘀汤不同剂量组血浆中AT-Ⅲ含量降低、PC含量升高(P<0.01). 结论 血府逐淤汤不同剂量组抗凝机制可能通过与AT-Ⅲ结合而灭活凝血因子,促进了PC对凝血因子的灭活,从而发挥抗凝作用.

Effects of Xuefu Zhuyu Decoction on the Anticoagulant System of Arterial Thrombosis Model Rats

Objective To analyze the effects of Xuefu Zhuyu Decoction in different dose groups on the arterial thrombosis caused by FeCl3 and the concentration of antithrombin-Ⅲ(AT-Ⅲ) and protein C (PC) in plasma of rats. Methods 60 SD rats, half male and half female, were randomly divided into blank group, model group, aspirin group, Xuefu Zhuyu Decoction of high, medium and low dose group. All rats were administrated continuously for 14 days by gavage. Except the blank group, the arterial thrombosis rat models were induced by FeCl3 applied onto the carotid artery. The weight of thrombosis and the concentration of AT-Ⅲ and PC in plasma of rats were measured. Results The model group has obvious thrombosis indicating a successful model. Compared with the model group, the thrombus weight of Xuefu Zhuyu Decoction in high, medium and low dose group and aspirin group were significantly decreased, in which the weight of medium dose group was the lightest (P<0.01). The AT-Ⅲ content of Xuefu Zhuyu Decoction in different dose group were significantly lower, while PC content were significantly increased (P<0.01). Conclusion Anti-thrombotic action of Xuefu Zhuyu Decoction in different dose groups might concern inactivating coagulation factors by binding with AT-Ⅲ or accelerating inactivation of coagulation factors by PC.