| 乙型肝炎病毒(adw_2)亚型全基因组的分子克隆 |
| |
| 引用本文： | 张振生,买凯.乙型肝炎病毒(adw_2)亚型全基因组的分子克隆[J].郑州大学学报(医学版),1988(4). |
| |
| 作者姓名： | 张振生 买凯 |
| |
| 作者单位： | 河南省医学科学研究所
(张振生)，河南省医学科学研究所(买凯) |
| |
| 摘 要： | 应用基因克隆技术,使高纯度的HBV DNA(adw_2)的EcoRI酶切片段分别与经同种限制性内切酶切割的PBR322和pUC18质粒DNA片段重组,转化到相应的E.coli受体菌中,经筛选和鉴定,得到了含有完整HB V(adw_2)基因组的分子克隆。
|
| 关 键 词： | 基因克隆 HBV DNA 限制性内切酶 质粒DNA 分子克隆 |
Cloning of hepatitis B virus genome (subtype adw_2) |
| |
| Zhang Zhenseng et al Henan Institute of Medical Sciences.Cloning of hepatitis B virus genome (subtype adw_2)[J].Journal of Zhengzhou University: Med Sci,1988(4). |
| |
| Authors: | Zhang Zhenseng Henan Institute of Medical Sciences |
| |
| Institution: | Zhang Zhenseng et al Henan Institute of Medical Sciences |
| |
| Abstract: | The highly purified hepatitis B virus (HBV) DNA (adw_2) was digested with EcoRI, and the fragment was recombinated with EcOR I linerized pBR 322 andpUC 18 by use of the techniques of gene cloning. The recombinant plasmids ofpBR 322-HBV DNA and pUC 18-HBV DNA were then transformed into E. coliHB 101 and JM83 separately. Through further screening and characterization, wegot the cloning strains which contain the complete genome of HBV DNA(adw_2). |
| |
| Keywords: | gene cloning HBV DNA restriction endonuclease plasmid DNA molecular cloning |
| 本文献已被 CNKI 等数据库收录！ |
