纳米银溶液及其凝胶的体外抗菌作用研究

目的:探讨纳米银溶液及纳米银凝胶的体外抗菌作用,为将纳米银开发成烧烫伤外用抗感染药提供依据。方法:以磺胺嘧啶银为阳性对照,采用试管二倍稀释法考察纳米银溶液及纳米银凝胶对铜绿假单胞菌、金黄色葡萄球菌、大肠埃希菌、变形杆菌、沙雷氏菌、不动杆菌、表皮葡萄球菌、肠球菌、肺炎克雷伯菌、白色念珠菌等10种病菌的最低抑菌浓度。结果:纳米银凝胶、纳米银溶液及磺胺嘧啶银对上述10种细菌的最低抑菌浓度分别为2·5～10、4～32、32～64μg·mL-1,纳米银溶液及其凝胶的抗菌效果明显优于磺胺嘧啶银。结论:体外试验中纳米银溶液及其凝胶抗菌谱广、抗菌作用强,为其用于治疗烧伤、烫伤等感染提供了一定试验依据。

Study on the in Vitro Antibacterial Effects of Silver Nanoparticle Solution and Silver Nanoparticle Gel

OBJECTIVE:To investigate the in vitro antibacterial effects of silver nanoparticle solution and silver nanoparticle gel in order to provide theoretical basis for external application of silver nanoparticles for anti-infection of burn and scald.METHODS:Using sulfadiazine silver as positive control,the minimum inhibitory concentrations of silver nanoparticles solution and silver nanoparticle gel to Pseudomonas aeruginosa,Staphylococcus aureus,Escherichia coli,Bacillus proteus,Serratia marcescens,Pseudomonas spec,Staphylococcus epidermidis,Enterococcus,Klebsiella pneumoniae,Candida albicans were determined by tube double dilution method.RESULTS:The minimum inhibitory concentrations of silver nanoparticle solution,silver nanoparticle gel and sulfadiazine silver to above 10 bacterials were 2.5～10 μg·mL-1,4～32 μg·mL-1,32～64 μg·mL-1.Antibacterial effects of silver nanoparticles solution and silver nanoparticle gel were better than sulfadiazine silver.CONCLUSION:The two forms of silver nanoparticles have broad antibacterial spectrum and strong antibacterial activity in vitro,which provide theoretical basis for external application of silver nanoparticles for anti-infection of burn and scald.