系统性红斑狼疮患者外周血淋巴细胞表型分析

目的 了解Ｔ淋巴细胞亚群、Ｂ细胞及自然杀伤细胞（ＮＫ细胞）在活动期ＳＬＥ患者中的改变，探讨这些免疫细胞在ＳＬＥ发病机理中的作用方法 采用微量微量全血标记免疫荧光染色和流式细胞仪，对３８例活动期ＳＬＥ患者及２０例正常人的外周血淋巴细胞表型进行检测。结果 ３８例活动期ＳＬＥ患者ＣＤ４＾＋细胞和ＣＤ４＾＋／ＣＤ８＾＋比值降低（Ｐ〈０．０１），ＣＤ３＾＋细胞、ＣＤ４＾＋细胞与正常对照组比较无统计学差异（Ｐ

Phenotypic analysis of peripheral blood lymphocytes in patients with systemic lupus erythematosus

Objective To investigate the significance of changes in T lymphocyte subsets, B cells and natural killer cells (NK cells) of peripheral blood in patients with systemic lupus erythematosus (SLE). Methods Phenotypic analysis of peripherial blood lymphocytes was conducted in 38 cases of active SLE and 20 healthy controls by using doublestaining immunofluoresence and flow cytometry. Results Decrease in CD + 4 cells and CD + 4/CD + 8 was observed in SLE patients ( P<0.01 ). No statistically significant differences were shown between two groups in CD + 3 cells and CD + 8 cells ( P>0.05 ). CD + 16 CD + 56 cells (NK cells) significantly decreased and CD + 19 cells(B cells) significantly increased in SLE patients, as comparing with the control group ( P<0.001 ). Conclusion Immunologic disorder is present in the peripheral blood of SLE patients. The changes of peripheral T lymphocyte subsets and decrease of NK cells, which lead to proliferation of B cells, may play an important role in the development of SLE.