外源性p21WAF1/CIP1基因转染对人胶质瘤细胞生长和细胞周期的影响 |
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引用本文: | 王占祥,章翔,费舟,张剑宁,付洛安,刘卫平,贺晓生,王彦刚,梁景文,王西玲. 外源性p21WAF1/CIP1基因转染对人胶质瘤细胞生长和细胞周期的影响[J]. 医学争鸣, 2003, 24(22): 2030-2033 |
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作者姓名: | 王占祥 章翔 费舟 张剑宁 付洛安 刘卫平 贺晓生 王彦刚 梁景文 王西玲 |
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作者单位: | 第四军医大学西京医院全军神经外科研究所,陕西,西安,710033 |
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基金项目: | 军队医药卫生科研项目,01MA187, |
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摘 要: | 目的:探讨外源性p21^WAF1/CIP1基因对胶质瘤细胞生长和细胞周期的影响.方法:采用脂质体介导法将p21^WAF1/CIP1基因质粒转导人人胶质瘤细胞系SHG44细胞,然后用电镜、流式细胞仪等技术对细胞形态、生长及细胞周期进行观察。结果:酶切电泳和斑点杂交显示p21^WAF1/CIP1基因成功的转染至SHG44细胞。转染p21^WAF1/CIP1基因的细胞光镜及电镜下可见凋亡产生;生长速度明显慢于亲代细胞,其倍增时间约是对照细胞的二倍;细胞周期发生明显改变,G1期明显增多,S期明显减少.结论:外源性p21^WAF1/CIP1基因对SHG44细胞的生长和细胞周期有明显的抑制作用,并可诱导细胞凋亡产生。
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关 键 词: | 外源性p21^WAF1/CIP1基因 神经胶质瘤 肿瘤细胞 细胞生长 细胞周期 脱噬作用 |
文章编号: | 1000-2790(2003)22-2030-04 |
修稿时间: | 2003-09-08 |
Effects of exogenous p21WAF1/CIP1 gene on the growth and cell cycle of human glioma cells |
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Abstract: | AIM: To explore the effects of exogenous p21 WAF1/CIP1 gene on the growth and cell cycle of glioma cells. METHODS: The whole length of p21 WAF1/CIP1 cDNA was transfected into SHG44 glioma cells by lipofectin method. The morphology, growth and cell cycle of cells were observed by electron microscope and flow cytometry. RESULTS: Electrophoresis and dot hybridization indicated that p21 WAF1/CIP1 gene had been successfully transfected into SHG44 glioma cells. Under light and electron microscope, apoptosis was found in p21 WAF1/CIP1 infected cells. The growth speed of p21 WAF1/CIP1 infected cells decreased markedly. The average multiplication time almost doubled compared with that of the uninfected cells. Analysis of cell cycle by flow cytometry showed that 90.8% of the infected cells were in G1 phase and 8.8% in S phase, while 58.7% of the control SHG44 cells were in G1 phase and 33.1% cells in S phase. CONCLUSION: Exogenous p21 WAF1/CIP1 gene can inhibit the growth and cell cycle of human glioma cells and induce their apoptosis. |
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Keywords: | p21 WAF1/CIP1 gene glioma cell cycle apoptosis |
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