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| 真核表达载体pcDNA3.1-MAGE-1的构建 | |
| 引用本文： | 何炜,张朝,章茜,赵国强,董子明. 真核表达载体pcDNA3.1-MAGE-1的构建[J]. 郑州大学学报(医学版), 2005, 40(4): 586-587 |
| 作者姓名： | 何炜 张朝 章茜 赵国强 董子明 |
| 作者单位： | 1. 郑州大学基础医学院生理学教研室,郑州,450052 2. 郑州大学基础医学院微生物学与免疫学教研室,郑州,450052 3. 郑州大学基础医学院病理生理学教研室,郑州,450052 |
| 基金项目： | 河南省医学科技创新人才工程项目20043008 |
| 摘 要： | 目的：构建含MAGE—1基因的重组真核表达质粒。方法：用RT—PCR方法从人肝细胞肝癌组织中扩增出MAGE-1基因cDNA序列，克隆至pGEM—T载体，测序证实基因碱基序列无误后，再克隆至真核表达载体pcDNA3.1( )，构建了pcDNA3.1—MAGE—1重组质粒。结果与结论：RT—PCR获得长度为927bp的阳性产物，经T载体和pcDNA3.1( )真核表达载体克隆、酶切鉴定及序列分析后。证实真核表达质粒pcDNA3．1—MAGE—1构建成功。 |
| 关 键 词： | MAGE—1 pcDNA3．1（＋） 真核表达载体 |
| 修稿时间： | 2005-03-10 |
Construction of pcDNA3.1-MAGE-1 expression vector |
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| HE Wei,ZHANG Zhao,ZHANG Qian,ZHAO Guoqiang,DONG Ziming. Construction of pcDNA3.1-MAGE-1 expression vector[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(4): 586-587 | |
| Authors: | HE Wei ZHANG Zhao ZHANG Qian ZHAO Guoqiang DONG Ziming |
| Affiliation: | HE Wei 1),ZHANG Zhao 1),ZHANG Qian 1),ZHAO Guoqiang 2),DONG Ziming 3) 1)Department of Physiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 4500522)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 3)Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim: To construct the pcDNA3.1 GAAB2 MAGE GAAB2 1 expression vector.Methods: The mRNA of MAGE GAAB2 1 gene was isolated from human hepatic cell carcinoma tissue.The cDNA was amplified using RT GAAB2 PCR,and cloned with pGEM GAAB2 T vector.The MAGE GAAB2 1 gene was inserted into the vector pcDNA3.1(+) after sequencing . Results and Conclusion: A 927 bp fragment was obtained by RT GAAB2 PCR. After double enzyme digestion, the MAGE GAAB2 1 gene was inserted into pcDNA3.1(+)vector. The result showed that the eukaryotic expression vector pcDNA3.1 GAAB2 MAGE GAAB2 1 was constructed successfully. |
| Keywords: | MAGE-1 pcDNA3.1(+) eukaryotic expression vector |
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