SFN联合5-Fu对ACC—M细胞增殖抑制的实验研究

目的：评价莱菔硫烷（SFN）与5-氟尿嘧啶（5-Fu）对人高转移唾液腺腺样囊性癌细胞（ACC—M）生长的抑制作用，同时检测参与诱导抗癌药物耐药性的核因子KB（NF—kB）的表达。方法：中位效应法评价SFN与5-Fu联用对ACC—M细胞增殖的影响．NF—kBp65表达采用Western印迹法检测。采用SPSS10．0软件包对所得数据进行t检验和方差分析。结果：SFN与5-Fu单独作用于ACC—M时的中位抑制浓度分别为（38．58±4．97）umol／L和（120．90±12．96）umol／L。两者联合使用时，对ACC—M细胞生长呈现协同抑制作用，并且引起细胞核NF—kBp65表达显著降低。与对照组相比．两药联合使用时．NF—kBp65的表达约降低5倍，P〈0．05。结论：SFN与5-Fu联合使用可协同抑制ACC—M细胞增殖．该作用与SFN、5-Fu协同抑制细胞核NF—kBp65的表达有关。

Combined inhibitory effect of sulforaphane and 5-fluorouracil on cell proliferation in human ACC-M cells

PURPOSE： To evaluate the growth-inhibitory effect of sulforaphane （SFN） and 5-fluorouracil （5-Fu） against the proliferation of human high metastatic cell line of salivary gland adenoid cystic carcinoma （ACC-M）. The expression of nuclear factor kappa B （NF-KB） inducing resistance to anticancer chemotherapeutic agent was also detected. METHODS： The combination effect of SFN and 5-Fu was quantitatively determined using the method of median effect principle and the combination index. The NF-KB p65 expression after treatment with the SFN-5-Fu combination was also evaluated by Western blotting analysis. Statistical comparison was performed by Student＇s t test and analysis of variance （ANOVA）, using SPSS 10.0 Software Package. RESULTS： The ICes for SFN and 5-Fu in ACC-M ceils were 38.58±4.97 umol/L and 120.90±12.96umol/L, respectively. Treatment of ACCs cells with SFN and 5-Fu in combination, led to synergistic inhibition on cell growth and a decreased expression of NF-KB p65 protein. When the two drugs were combined, the level of NF-KB p65 protein expression was decreased almost 5-fold （P〈0.05）. CONCLUSION： The results demonstrate synergism between SFN and 5-Fu against the proliferation of ACC-M cells, which is associated with the decreased expression of NF-KB p65 protein.Supported by Postdoctorate Foundation of Heilongjiang Province（Grant No.LRD06-185）.