高效液相色谱-库仑电化学检测法定量测定小鼠血浆中CTN986

 目的建立血浆中CTN986的定量测定方法,为药物代谢研究提供技术手段。方法采用HPLC-库仑电化学色谱方法,Diamonsil^TM(钻石)C₁₈(,4.6 mm×200 mm,5μm)色谱柱,0.2 mol·L^-1磷酸二氢钠-甲醇(60∶40)为流动相,CoulochemⅢ电化学检测器,Model 5010 Analytical cell为检测手段,以峰面积定量。结果检测下限为150μg·L^-1,定量下限为390μg·L^-1,方法线性范围为0.390～12.5,12.5～50 mg·L^-1;在1.563,6.25,12.5,25 mg·L^-14种质量浓度平均回收率为53.43%;日内、日间平均RSD为1.03%,2.28%。结论该方法灵敏度高,方法操作简便,可满足药动学研究的要求。

Determination of CTN986 in Mice Plasma by HPLC-ECD

OBJECTIVE To develop a method for the determination of CTN986 in mice plasma.METHODS The separation was performed on a Diamonsil^TM C₁₈ column(4.6 mm×200 mm,5 μm) with the mobile phase consisting of 0.2 mol·L^-1 sodium dihydrogen phosphate-methanol(60∶40).The compound was detected by Coulochem Ⅲ electrochemistry detector equiped with Model 5010 Analytical cell.RESULTS The limit of detection was 150 μg·L^-1 and the limit of quantification was 390 μg·L^-1.The calibration curves were linear in the range of 0.390～12.5 and 12.5～50 mg·L^-1.The average extraction recovery was 53.43%,the RSDs of inter-day and intra-day validation were below 3.0%.CONCLUSION The method is sensitive,convenient and applicable for pharmacokinetic study.