葛根素对糖尿病大鼠血脂和主动脉基膜粘连蛋白B1 mRNA表达的影响

目的：观察葛根素对糖尿病大鼠血脂和主动脉基膜粘连蛋白B1 mRNA表达的影响.方法：利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型, 将实验用SD大鼠随机分正常对照组、糖尿病组、葛根素治疗组.治疗12周,观察治疗期间及治疗后大鼠的一般状况、血糖（BS）、甘油三脂（TG）、胆固醇（TC）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）、糖化血红蛋白（HbA1c）、糖化低密度脂蛋白（G-LDL）;分离主动脉,HE染色及原位杂交检测主动脉内膜基膜粘连蛋白B1 mRNA表达.结果：造模2组大鼠均出现血脂异常及主动脉病理形态改变;葛根素能改善基本状况,降低糖尿病大鼠的甘油三脂、胆固醇、低密度脂蛋白、糖化血红蛋白和糖化低密度脂蛋白（P＜0.05）;升高高密度脂蛋白（P＜0.05）;降低主动脉基膜粘连蛋白B1 mRNA表达（P＜0.05）.结论：葛根素具有确切的主动脉保护作用.

Effects of puerarin on blood lipid and expression of aorta laminin B1 mRNA in streptozotocin-induced diabetic rats

AIM: To observe the effects of puerarin on blood lipid and expression of aorta laminin B_1 mRNA in streptozotocin-induced diabetic rats. METHODS: Diabetic nephropathy rats were induced by intraperitoneal injection of STZ, and the experimental rats were divided into normal control group, model group, and puerarin group. During and after the treatment for 12 weeks, the general state, blood suger(BS), triglyceride(TC), cholesterol, low density lipoprotein(LDL-C), high density lipoprotein(HDL-C), glycosylated hemoglobin(HbA1c) and glycosylated low density lipoprotein(G-LDL) were detected. Aorta alteration of tissue morphology was observed by H.E staining, and the expressions of laminin B1 mRNA were determined by in situ hybridization analysis. RESULTS: Diabetes mellitus and aorta lesion occurred in the two model groups. Puerarin could improve the general state, decrease the level of triglyceride(P< 0.05 ), serum cholesterol (P< 0.05 ), low density lipoprotein(P< 0.05 ), glycosylated hemoglobin(P< 0.05 ), and glycated low density lipoprotein(P< 0.05 ), and increase the level of high density lipoprotein(P< 0.05 ). The expression of laminin B_1 mRNA in the aorta were significantly inhibited by Puerarin (P< 0.05 ). CONCLUSION: Puerarin have certain protective effects on aorta in rats.