氯化锂对K562白血病细胞增殖和凋亡的影响

目的 :研究氯化锂 (LiCl)在体外对K5 6 2白血病细胞增殖及凋亡的影响。方法 :采用液体培养试验 ,四唑盐 (MTT)比色试验 ,集落培养试验为指标观察LiCl对K5 6 2细胞增殖的影响 ,采用DNA片段凝胶电泳及细胞形态学改变为指标检测细胞凋亡。结果 :①不同浓度的LiCl(10～ 5 0mmol/L)对K5 6 2细胞具有抑制增殖的作用 ,这种增殖抑制作用呈剂量依赖关系。②在LiCl(30mmol/L)作用下 ,K5 6 2细胞形态学上可见凋亡细胞 ,且DNA琼脂糖凝胶电泳谱可见“梯形带”(DNAladder)。结论 :LiCl能抑制K5 6 2白血病细胞增殖和诱导其凋亡。

Effects of lithium chloride on the proliferation and apoptosis of K562 leukemia cells

Objective To investigate the effects of lithium chloride on the proliferation and apoptosis of human leukemia cell line (K562). Methods The effects of lithium chloride on the proliferation of K562 leukemia cells were evaluated by liquid culture assay, MTT assay,and colony formation assay. The effects of lithium chloride on the apoptosis of K562 leukemia cell were verified by cellular morphology and the agarose gel electrophoresis of DNA fragments. Results ①The proliferation of K562 leukemia cells was inhibited by lithium chloride (10～50 mmol/L) in a dose-dependent manner. ②With lithium chloride (30 mmol/L), the apoptosis cells could be observed in cellular morphology, and DNA ladder was also observed in agarose gel electrophoresis. Conclusion Lithium chloride can inhibit the proliferation of K562 cells and cause the apoptosis of K562 cells.