| 脑泰方提取物对局灶性脑缺血大鼠血管内皮生长因子表达的影响 |
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| 引用本文： | 陈懿,王国佐,葛金文.脑泰方提取物对局灶性脑缺血大鼠血管内皮生长因子表达的影响[J].中华中医药杂志,2009(2). |
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| 作者姓名： | 陈懿 王国佐 葛金文 |
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| 作者单位： | 湖南中医药大学生理学教研室;湖南中医药大学科技处; |
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| 基金项目： | 湖南省自然科学基金资助项目(No.08JJ4008); 湖南省教育厅科研基金重点项目(No.07A050); 湖南省教育厅科研基金青年项目(No.06B068); 湖南省卫生厅科研基金资助项目(No.B2006-133) |
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| 摘 要： | 目的:探讨脑泰方提取物对大鼠局灶性脑缺血后血管内皮生长因子(VEGF)及VEGF mRNA的影响。方法:30只SD大鼠随机分为假手术组、模型组、脑复康组、脑泰方低、中、高剂量组。通过月桂酸钠损伤血管内皮细胞来复制局灶性脑缺血模型,采用免疫组化和RT-PCR法检测各组大鼠脑内VEGF阳性细胞数和VEGF mRNA的表达,同时评价大鼠神经功能。结果:脑缺血后,药物组神经功能症状得到改善,尤以脑泰方中剂量组变化最显著(P<0.01);假手术组大鼠脑内有少量VEGF阳性细胞,脑缺血后VEGF阳性细胞表达增加,与模型组比较,脑泰方中剂量组VEGF阳性细胞数明显增多(P<0.01),而且VEGF mRNA的表达也显著加强(P<0.01)。结论:脑泰方提取物增强大鼠局灶性脑缺血后VEGF及VEGF mRNA的表达,可能是其抗脑缺血的作用机制之一。
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| 关 键 词： | 局灶性脑缺血 血管内皮生长因子(VEGF) 脑泰方提取物 |
Effects of Naotaifang extracts on expression of VEGF after focal cerebral ischemia in rats |
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| CHEN Yi,WANG Guo-zuo,GE Jin-wen.Effects of Naotaifang extracts on expression of VEGF after focal cerebral ischemia in rats[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(2). |
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| Authors: | CHEN Yi WANG Guo-zuo GE Jin-wen |
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| Institution: | CHEN Yi1,WANG Guo-zuo2,GE Jin-wen2(1Department of Physiology,Hunan University of Chinese Medicine,Changsha 410208,China,2Department of Science , Technology,China) |
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| Abstract: | Objective:To approach the effect of Naotangfang extracts on expression of vascular endothelial growth factor(VEGF) after focal cerebral infarct in rats. Methods:30 Sprague-Dawley(SD) rats were randomly divided into 6 groups:sham operation group,model group,Naotaifang extract groups(low,middle,and high dosage) ,and Naofukang group. Some indexes were detected such as neurological scores,VEGF mRNA expression by RT-PCR,and the number of VEGF positive cells by immunohistochemical methods. Results:The symptoms in... |
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| Keywords: | Focal cerebral ischemia VEGF Naotaifang extract |
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