| 免疫印迹法与免疫荧光法检测寻常型天疱疮自身抗体的比较 |
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| 引用本文： | 李卫平,潘萌,薛峰,郑捷.免疫印迹法与免疫荧光法检测寻常型天疱疮自身抗体的比较[J].现代免疫学,2003,23(1):43-44. |
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| 作者姓名： | 李卫平 潘萌 薛峰 郑捷 |
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| 作者单位： | 上海第二医科大学附属瑞金医院皮肤科,上海,200025 |
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| 摘 要： | 比较免疫印迹法与免疫荧光法对检测寻常型天疱疮抗体的临床应用价值。利用重组寻常型天疱疮抗原 (PVAEC1 2 )进行寻常型天疱疮 (PV )患者的免疫印迹法 (IBT )检测 ,并同时应用直接免疫荧光法 (DIF )和间接免疫荧光法 (IIF )检测 2 5例PV患者。IBT 2 1例阳性 (84 % ) ,DIF 2 0例阳性 (80 % ) ,IIF 18例阳性 (72 % )。正常对照 10例均为阴性。免疫荧光技术在PV的诊断与鉴别诊断中有其重要作用。利用重组PVAEC1 2行IBT以检测PV患者血清中抗PVA抗体 ,与免疫荧光法敏感性无统计学差异
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| 关 键 词： | 免疫印迹法 直接免疫荧光法 间接免疫荧光法 寻常型天疱疮抗体 |
| 文章编号： | 1001-2478(2003)01-0043-02 |
| 修稿时间： | 2002年3月27日 |
Comparison of Immunoblot and Immunofluorescence in Detecting Pemphigus Vulgaris Antibodies |
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| LI Wei ping,PAN Meng,XUE Feng,ZHENG Jie.Comparison of Immunoblot and Immunofluorescence in Detecting Pemphigus Vulgaris Antibodies[J].Current Immunology,2003,23(1):43-44. |
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| Authors: | LI Wei ping PAN Meng XUE Feng ZHENG Jie |
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| Abstract: | To compare the clinical value of immunoblot and immunofluorescence in detecting pemphigus vulgaris antibody,we used recombinant pemphigus vulgaris antigen (PVA EC1 2) fusion protein as substrate to detect autoantibodies in 25 cases of sera from patients with PV by immunoblot,direct and indirect immunofluorescence 21 cases (84%),20 cases (80%),and 18 cases (72%) were positive in immunoblot,in direct and in indirect immunofluoresence respectively All the normal persons were negative The results showed that immunofluoresence was important in diagnosing and identification diagnosing to PV patients Using the PVA EC1 2 fusion protein as substrate to detect autoantibodies of sera from patients with PV by immunoblot had a similar sensitivity to immunofluorescence |
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| Keywords: | immunoblotting direct immunofluorescence indirect immunofluorescence pemphigus vulgaris antibodies |
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