花类药材金银花精准煮散饮片的质量评价

目的：饮片质量均一性是中药稳定临床疗效的基础。本文基于成分分析与DNA分子鉴定手段，考察金银花精准准煮散饮片与市售原饮片质量差异，探索精准煮散饮片的质量控制方法。方法：应用ITS2序列作为DNA条形码对金银花饮片进行鉴定；对比金银花市售原饮片及精准煮散饮片的干膏收率；采用HPLC-DAD法进行3批次饮片混合粉碎前后指标成分含量、指纹图谱及相似度评价。结果：金银花煮散饮片出膏率及指标成分绿原酸含量均略高于市售饮片；市售饮片绿原酸的批间溶出量有明显的差异性，RSD为11.93%；混合制成煮散饮片后差异降低，RSD为8.29%。指纹图谱相似度结果显示，混合粉碎后指纹图谱相似度提高，标定共有峰7个，峰面积均有不同程度提高。结论：金银花精准煮散饮片与市售原饮片基本属性相一致，但煮散饮片的出膏率、成分溶出及质量均一性均有明显提高，表明煮散饮片有较好的临床应用优势。

A Study on Quality of the Precise Powder Decoction Pieces of Medicinal Flowers Lonicerae japonicae Flos

This study aimed at evaluating the quality of the precise powder decoction pieces(PPDP)of L.japonicae Flos(LJF)compared with the traditional commercial slices with chemical fingerprint methods and DNA molecular identification technology.Different specifications of PPDP were prepared,their dry extract contents were in contrast with that of commercial slices.The three batches of commercial slices were collected,and the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were studied by HPLC-DAD and DNA sequence alignment.As a result,the paste rate of PPDP was slightly higher than that of the traditional commercial slices.The dissolution of chlorogenic acid of PPDP was higher than that of the traditional commercial slices.RSD of inter-assay dissolutions of chlorogenic acid of commercial slices was 11.93%,which was reduced to 8.29% after mixing and preparing into PPDP.The fingerprint showed that the slimilarity of the fringerprint of the mixed and powdered LJF was elevated with 7 common peaks.All the common peaks were increased at different levels.In conclusion,compared with traditional commercial slices of LJF,PPDP apparently improved the dissolution rate and the quality uniformity,indicating that the boiled powder of CRP obviously presented vantages in clinic.