| 聚己内酯电纺纤维支架材料对骨髓基质细胞增殖及分化的影响 |
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| 引用本文： | 贾骏,段嫄嫄,周建学,张少锋. 聚己内酯电纺纤维支架材料对骨髓基质细胞增殖及分化的影响[J]. 医学争鸣, 2007, 28(13): 1153-1155 |
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| 作者姓名： | 贾骏 段嫄嫄 周建学 张少锋 |
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| 作者单位： | 第四军医大学口腔医学院修复科,陕西,西安,710033;第四军医大学口腔医学院设备科,陕西,西安,710033 |
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| 基金项目： | 国家自然科学基金，陕西省自然科学基金 |
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| 摘 要： | 目的:观察聚己内酯(PCL)电纺纤维支架材料对骨髓基质细胞(BMSCs)增殖及分化特点的影响,评价其作为骨组织工程的支架材料的应用前景.方法:将兔BMSCs与PCL电纺纤维支架材料在培养板内共培养.采用形态学观察、MTT法及ALP检测等方法检测BMSCs在PCL电纺纤维表面的粘附、增殖和分化能力.结果:体外培养的BMSCs能在PCL电纺纤维表面正常粘附和增殖,并且表达较高的碱性磷酸酶活性.结论:PCL电纺纤维适合作为支架材料应用于BMSCs为种子细胞的组织构建.
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| 关 键 词： | 电纺纤维 聚己内酯 骨髓基质细胞 |
| 文章编号： | 1000-2790（2007）13-1153-03 |
| 修稿时间： | 2007-03-292007-04-10 |
Effect of polycaprolactone-based electrospun nanofibrous framework on proliferation and differentiation of bone marrow stromal cells in vitro |
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| JIA Jun,DUAN Yuan-Yuan,ZHOU Jian-Xue,ZHANG Shao-Feng. Effect of polycaprolactone-based electrospun nanofibrous framework on proliferation and differentiation of bone marrow stromal cells in vitro[J]. Negative, 2007, 28(13): 1153-1155 |
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| Authors: | JIA Jun DUAN Yuan-Yuan ZHOU Jian-Xue ZHANG Shao-Feng |
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| Abstract: | AIM: To evaluate the effect of polycaprolactone (PCL)-based electrospun nanofibers on proliferation and differentiation of bone marrow stromal cells (BMSCs). METHODS: BMSCs were cultured in vitro and implanted on PCL-based electrospun nanofibers. The proliferation and differentiation of BMSCs were detected through scanning electron microscopy (SEM), MTT assay and the detection of alkaline phosphatase (ALP) activity. RESULTS: BMSCs could adhere to and proliferate on the surface of PCL-based electrospun nanofibers. BMSCs had a higher ALP activity. CONCLUSION: PCL-based electrospun nanofiber is a suitable framework material for BMSCs to fabricate tissue engineered bone. |
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| Keywords: | electrospun nanofibers polycaprolactone bone marrow stromal cells |
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