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| 临床分离产IMP-1型金属酶非发酵菌的耐药机制研究 | |
| 引用本文： | 程曦,贾文祥,杜宝中,曾蔚,康梅,张再蓉,谢轶,杨维青,范红,陶传敏,张磊. 临床分离产IMP-1型金属酶非发酵菌的耐药机制研究[J]. 生物医学工程学杂志, 2007, 24(4): 876-879 |
| 作者姓名： | 程曦 贾文祥 杜宝中 曾蔚 康梅 张再蓉 谢轶 杨维青 范红 陶传敏 张磊 |
| 作者单位： | 1. 成都医学院医学检验系,610081 2. 四川大学,华西基础医学与法医学院,微生物学教研室,成都,610041 3. 四川大学,华西医院,实验医学科,成都,610041 |
| 摘 要： | 从临床收集耐亚胺培南的铜绿假单胞菌和鲍曼不动杆菌共50株,进行头孢他啶和2-巯基乙醇的表型协同试验(CAZ ME),然后进行金属酶IMP-1基因的PCR检测。选取IMP-1阳性株测序,用PCR方法检测有无一类整合子基因(IntI1)。表型的检测发现有28株为协同阳性,其中铜绿假单胞菌27株,鲍曼不动杆菌1株。PCR和测序检测出其中一株铜绿假单胞菌含有IMP-1基因,同时也含有IntI1基因。首次在中国西部地区发现产IMP-1型金属酶、同时也含有一类整合子的铜绿假单胞菌,对于临床上研究细菌的耐药性传播具有重要意义。 |
| 关 键 词： | 整合子 非发酵菌 |
| 修稿时间： | 2006-04-042006-10-23 |
The Drug-resistant Mechanism of Clinical Non-fermenting Bacilli Producing IMP-1 Metalloenzyme |
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| Cheng Xi,Jia Wenxiang,Du Baozhong,Zeng Wei,Kang Mei,Zhang Zairong,Xie Yi,Yang Weiqing,Fan Hong,Tao Chuanmin,Zhang Lei. The Drug-resistant Mechanism of Clinical Non-fermenting Bacilli Producing IMP-1 Metalloenzyme[J]. Journal of biomedical engineering, 2007, 24(4): 876-879 | |
| Authors: | Cheng Xi Jia Wenxiang Du Baozhong Zeng Wei Kang Mei Zhang Zairong Xie Yi Yang Weiqing Fan Hong Tao Chuanmin Zhang Lei |
| Affiliation: | 1.Department of Microbiology, West China School of Basic Medical Science and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2 .Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China |
| Abstract: | A total of 50 clinical imipenem-resisitant isolates of Pseudomonas aeruginosa and Acinetobacter baumannii were subjected to the ceftazidime-2-mercaptoethanol -double-disk synergy test and to the PCR assays with primers specific for blaIMP-1.After the process of sequencing the positive one to identify the results, PCR analysis was conducted with primers specific for class 1 integrons. For synergy test, 28 isolates gave positive results, among which were 27 Pseudomonas aeruginosa and Acinetobacter baumannii. Only one Pseudomonas aeruginosa was found to carry blaIMP-1, and blaInt1 at the same time. This is the first ascertainment of IMP-1 producing Pseudomonas aeruginosa isolate carrying blaIntI1 in West China,which is of significantce to the research on the clinical spread of these drug-resisitant genes. |
| Keywords: | IMP-1 |
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