| Abstract: | Some features of secretion of labeled somatotrophic hormone (STH) and prolactin in a 5-day monolayer culture of rat adenohypophyses were studied. Hormones were isolated from the culture medium by electrophoresis in polyacrylamide gel. Sodium-dibutyryl cyclic AMP and theophylline stimulated STH and prolactin secretion. Thyrotrophin releasing hormone (TRH) increased the rate of incorporation of L-leucine-14C into the cell proteins, stimulated prolactin secretion, but did not affect STH liberation. Somatostatin completely inhibited theophylline-induced STH secretion but did not affect prolactin secretion. The characteristics of formation of the labeled STH and prolactin pool in the cells and of the secretion of these hormones into the culture medium are discussed.Laboratory of Biological Standardization of Hormones, Institute of Experimental Endocrinology and Hormone Chemistry, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR N. A. Yudaev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 10, pp. 491–495, October, 1978. |
