树状细胞疫苗抑制肿瘤的实验观察

目的观察经肿瘤抗原致敏的树状细胞（DC）疫苗对荷瘤裸鼠的抑瘤作用。方法重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）、重组人白细胞介素4（rhIL-4）、重组人肿瘤坏死因子α（rhTNF-α）协同诱导人外周血单核细胞成为DC。Tea8113舌癌细胞可溶性抗原体外致敏DC，将同源人T淋巴细胞与之共同培养诱导出抗原特异性的细胞毒性T淋巴细胞（CTLs），共同接种至荷瘤裸鼠体内行免疫治疗。结果人外周血单核细胞在细胞因子协同诱导下可获得功能正常的DC，用致敏DC＋CTLs免疫治疗的荷瘤裸鼠与其他实验组相比，明显抑制肿瘤生长，肿瘤倍增时间显著提高（P〈0．05）。结论从人外周血来源的单核细胞在多种细胞因子作用下可诱导为功能正常的DC、冻融抗原体外致敏的DC，经其激活的肿瘤特异性CTLs对荷舌鳞癌裸鼠有明显的免疫治疗作用，可望成为口腔颌面部舌癌免疫治疗的新途径。

Inhibitory effect of dendritic cell vaccine on the growth of implanted tongue squamous cell carcinoma tumors in nude mice

OBJECTIVE: To investigate the inhibitory effect of dendritic cells (DCs) on the growth of the implanted tongue squamous cell carcinoma (SCC) tumors in nude mice. METHODS: Being induced from human peripheral blood monocytes (PBMCs) with rhGM-CSF, rhIL-4 and rhTNF-alpha, DCs were pulsed by Tca8113 cells lysates. Those DCs and T lymphocytes were co-cultured to induce specific cytotoxic T lymphocytes (CTLs). Immunotherapy was then performed after those DCs and CTLs were implanted into the tumor-bearing nude mice. RESULTS: DCs were induced from PBMCs with multiple cytokins. Compared with the control groups, the growth of tumors was significantly inhibited in the group that had been implanted with DCs and CTLs, and tumor doubling time also increased markedly (P < 0.05). CONCLUSIONS: DCs function normally after being induced from human PBMCs with multiple cytokins; DCs pulsed with tumor cell lysates and CTLs co-cultured with those DCs have a significant anti-tumor immune activity in nude mice.