| DHCC受体的纯化 |
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| 引用本文： | 孙宝栋,石育原.DHCC受体的纯化[J].吉林大学学报(医学版),1993(5). |
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| 作者姓名： | 孙宝栋 石育原 |
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| 作者单位： | 白求恩医科大学分子生物学中心实验室
(孙宝栋)，白求恩医科大学分子生物学中心实验室(石育原) |
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| 摘 要： | 本文报告了正常雄性家兔小肠粘膜细胞核提取液,经 38％饱和废硫酸铵沉淀、DEAE-纤维素层析、DNA-纤维素层析、羟基磷灰石层析、Sephadex G—200凝胶过滤,最终得到纯化5200倍的DHCC受体40μg,产率为9％。
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| 关 键 词： | 1 5—二羟维生素D_3受体 钙化醇/分离和提纯 受体/分离和提纯 |
Pruification of the receptor for 1α, 25-dihydroxyvitamin D_3 form rabbit intestine |
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| Sun Baodong,Shi Yuyuan.Pruification of the receptor for 1α, 25-dihydroxyvitamin D_3 form rabbit intestine[J].Journal of Jilin University: Med Ed,1993(5). |
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| Authors: | Sun Baodong Shi Yuyuan |
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| Institution: | Central Laboratory of Molecular Biology |
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| Abstract: | The technique in use in the purification of the rabbit intestinal receptor for 1, 25 (OH2) D3 included ammonium sulfate precipitation, DEAE-cellulose, DNA - cellulose, hydroxylapatite and Sephadex G -200 gel-exclusion Chromatography. This kind of purification scheme resulted in purification of the receptor protein by 5200 - fold with 9% yield.On analysis, the purified protein on polyacrylamide gel electrophoresis contains sodium dodecyl sulfate. Isoelectric focusing and isotachophoresis suggested that the protein is homogeneous and characterized by having a molecular weight of approximately 65 000 and a PI of 6.1. |
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| Keywords: | calcitriol/IP receptor/IP 1 25 (OH2) D3 receptor/IP intestine small/IP chromatography DEAE-cellulose/MT |
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