星点设计-响应面法优化维吾尔药刺山柑果总酚酸提取工艺

目的:优选维吾尔药刺山柑果总酚酸的提取工艺。方法:采用乙醇回流提取法,通过单因素试验确定提取次数,以乙醇体积分数、溶剂用量、提取时间为自变量,总酚酸提取量为因变量,通过对自变量各水平的多元线性回归及二项式拟合,利用响应面法优选刺山柑果总酚酸的提取工艺并进行预测分析。结果:最佳提取工艺为加15倍量70%乙醇提取2次,每次1 h;总酚酸提取量7.29 mg·g-1,与模型预测值(7.34 mg·g-1)的相对标准偏差0.68%。结论:星点设计-响应面法优选的刺山柑果总酚酸提取工艺简单、精密度高、可预测性好。

Optimization of Extraction Technology for Total Phenolic Acids from Capparis spinosa Fruits by Central Composite Design-response Surface Methodology

Objective: To optimize extraction process of total phenolic acids in Capparis spinosa fruits. Method: Independent variables were ethanol concentration,solvent amounts and extracting time,dependent variable was yield of total phenolic acids,levels of independent variables were regressed multiple linearity and fitted by binomial,response surface methodology was used to optimize extraction process,then compared both of observed and predicted values. Result: Optimum extraction progress was as follows:extracted 2 twice with 15 times the amount of 70% ethanol for 1 h per time;yield of total phenolic acids was 7.29 mg·g^-1,whose deviation was 0.68% by comparing with the predicated value(7.34 mg·g^-1). Conclusion: This optimized process was simple with high precision and good predictability.