一叶抗流感胶囊抑制甲型H1N1流感病毒感染的体内外研究

目的:研究一叶抗流感胶囊体内外抗甲型H1N1流感病毒的作用。方法:ICR小鼠随机分为对照组、利巴韦林组(0.07 g·kg-1)、抗病毒颗粒组(4.24 g·kg-1)、一叶抗流感胶囊(1.5,0.75,0.38 g·kg-1),采用甲型H1N1流感病毒(FM1,PR8)滴鼻感染小鼠造成肺炎模型,治疗给药于感染后连续ig 4 d,预防给药4 d后再感染病毒,计算肺指数、死亡率、死亡保护率、平均存活天数和生命延长率;体外Cytopathic effect(CPE)法观察一叶抗流感胶囊抗流感病毒的作用,犬肾上皮细胞(Madin-Darby canine kidney,MDCK)细胞密度为1×105个/mL,药物质量浓度为2.5,1.25,0.62,0.31 g·L-1,作用时间为48 h,每组4个样本。结果:与正常组比较,采用甲型H1N1流感病毒FM1和PR8病毒株感染小鼠后,治疗给药和预防给药下,病毒组肺指数均明显增高,治疗给药组的死亡率分别为90%和90%、预防给药组的死亡率为85%和55%,治疗给药组的平均存活天数分别为9.20 d和9.25 d、预防给药组的平均存活天数分别为9.35,11.60 d;一叶抗流感胶囊高、中剂量组治疗性给药后能明显降低流感病毒FM1株和PR8株感染后小鼠的肺指数(高剂量组1.03±0.28,0.88±0.13;中剂量组1.08±0.14,0.92±0.10);延长平均存活天数高剂量组(11.05±2.86),(11.20±2.86)d,中剂量组(10.95±2.78)d,(10.90±2.86)d,一叶抗流感胶囊高、中剂量组预防性给药明显延长平均存活天数(11.15±2.30),(11.75±2.24)d。一叶抗流感胶囊在体外对FM1毒株的IC50为0.89 g·L-1,治疗指数为6.75。结论:一叶抗流感胶囊对甲型H1N1流感亚型FM1株体内、体外均有明显的治疗作用;对PR8株仅体内治疗给药有效。

Activity of Yiye Anti-influenza Capsule Against Influenza A/H1N1 Virus in vitro and in vivo

Objective: To investigate the effect of Yiye Anti-influenza capsule (YYAFC) on the resistance to influenza A/H1N1 virus. Method: ICR mice were randomly divided into control group (0.5%CMC, 20 mL·kg^-1), ribavirin(0.07 g·kg^-1), influenza virus particles group (4.24 g·kg^-1) and YYAFC group(1.5, 0.75, 0.38 g·kg^-1). The pneumonic model of mice were infected by influenza A(H1N1) with nasal drops, mice were administrated after infection virus in treatment andprecautiongroups. The lung index, death rate, death protection rate, the average survival period and life prolongation were calculated;cytopathic effect(CPE) was used to evaluate the anti-influenza virus(H1N1) effect of YYAFC in vitro. Result: Compared to normal group, mice were infected with the A/H1N1 influenza virus (FM1 and PR8), the lung indexin treatment and precaution groupswassignificantly increased (P<0.01), death rate was 90% and 90% in treatment group and 85%and 55% in precautiongroup, respectively,the average survival period was 9.20 days and 9.25 days in treatment griups and 9.35 days and 11.60 days in precaution group. In the treatment group of high and medium dosage YYAFC, the lung index decreased significantly, while the life span of mice was prolonged.In the precaution group of high and medium dosage YYAFC, the life span of mice was prolongedsignificantly. The IC₅₀ of the sample inhibiting CPE caused by FM1 in vitro with doses of 0.89 g·L^-1 and the TI was 6.75. Conclusion: YYAFC showed inhibitory effect on influenza A virus FM1 stain in vivo and in vitro.