木蝴蝶提取物对酪氨酸酶的抑制及抗氧化作用

目的:研究木蝴蝶对酪氨酸酶的抑制及抗氧化作用。方法:以木蝴蝶醇提物的不同极性组分为研究对象,测定了木蝴蝶对酪氨酸酶的抑制作用,以及运用1,1-二苯-2-苦肼自由基(DPPH·)、羟基自由基(·OH)测定其抗氧化能力。结果:木蝴蝶醇提物的乙酸乙酯组分(EAO)、正丁醇组分(n-BO)、水提取组分(WO)对酪氨酸酶活性均有抑制作用,其半数抑制率(IC50)分别为3.081 4,8.525 3,22.750 8 g·L-1;DPPH·和·OH试验显示EAO,n-BO,WO具有较强的自由基清除作用,其清除自由基能力强弱次序均为EAO>n-BO>WO。结论:木蝴蝶具有良好的酪氨酸酶抑制活性及抗氧化作用,抑制酪氨酸酶活性的生成与其抗氧化能力具有正相关性;木蝴蝶醇提物的功能性成分主要分布在乙酸乙酯相中。

Effect on Anti-tyrosinase and Antioxidation Activities of Extract from Oroxylum indicum

Objective: To study anti-tyrosinase and antioxidation activities of extract from Oroxylum indicum. Method: This paper took different polar fractions of extract from the O. indicum ethanolic extract as research objects, measured its inhibition of tyrosinase and 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·) and the hydroxyl free radical (·OH) scavenging ability. Result: Its alcohol extract of ethyl acetate extract (EAO), n-butanol extract (n-BO) and water extract (WO) had inhibition to tyrosinaes, the values of IC₅₀ reached 3.081 4,8.525 3, 22.750 8 g·L^-1 respectively;EAO, n-BO,WO had stonger scavenging effect of DPPH·and·OH, the order of scavenging ability was EAO>n-BO>WO. Conclusion: O. indicum. had better tyrosinase inhibitory and antioxidation, its antioxidation ability had positive correlation with its anti-tyrosinase. Its functional components were mainly distributed in the ethyl acetate phase.