通莲汤对荷瘤裸鼠的抑瘤作用及机制研究

目的:探讨复方通莲汤对胃癌MGC803细胞荷瘤裸鼠的抑瘤作用及对肿瘤组织增殖细胞核抗原(PCNA)表达的影响,为其临床应用提供实验依据。方法:胃癌细胞株MGC803接种于裸鼠右前肢皮下。模型组ig生理盐水溶液10 mL·kg-1,阳性对照组ig消癌平片1.80 g·kg-1的药液10 mL·kg-1,复方通莲汤高、中、低剂量组分别ig 1.50,0.75,0.37 g·kg-1的药液10 mL·kg-1,各组连续给药3周,再连续观察3周,6周内连续测定裸鼠瘤体体积,最后脱臼处死全部裸鼠,分离瘤组织,计算抑瘤率,免疫组织化学法检测肿瘤组织PCNA表达。结果:通莲汤抑制瘤体生长与时间呈正相关,开始给药后第4周达抑瘤峰值;通莲汤高剂量组瘤重(1.55±0.68)g,抑瘤率为(58.40±1.56)%,与模型组比较有显著性差异(P<0.05);通莲汤可显著抑制PCNA表达(P<0.05)。结论:通莲汤可抑制裸鼠人胃癌MGC803细胞移植瘤生长,其抑瘤机制与下调肿瘤细胞PCNA蛋白表达有关。

Mechanism of Inhibiting Tumor Growth on Nude Mice Modeled with Gastric Cancer MGC803 Cells by Tonglian Decoction

Objective: The purpose of the research is to observe inhibition of Tonglian decoction (TD) on nude mice modeled with gastric cancer cells MGC803 and on proliferating cell nuclear antigen (PCNA) expression in tumor tissue, to provide experimental basis for clinical application of TD. Method: MGC803 cells were inoculated subcutaneously in right forelimbs of all mice. Mice in model group were treated with saline solution 10 mL·kg^-1 orally. Mice in positive control group were treated with xiaoaiping tablet at dosage of 10 mL·kg^-1, 1.80 g·kg^-1 (crude drug) of liquid. Mice in TD groups were subdivided as high (1.50 mg·kg^-1), medium (0.75 g·kg^-1) and low (0.37 g·kg^-1) dose group of drug liquid, with dosage of 10 mL·kg^-1. All mice were administrated for 3 weeks and were observed for another 3 weeks continuously. Tumor volumes were measured for 6 weeks every day from the beginning of MGC803 cells transplanted. All mice were killed for isolation of tumor tissue, inhibition ratio of tumor-weight measured. Finally, PCNA expression was examined with immunohistochemistry. Result: TD inhibits tumor growth with positive correlation with time, the inhibitory peak appeared at 4 weeks after medication;tumor weight in TD high-dose group was (1.55±0.68) g, and the inhibition ratio was (58.40±1.56)%, which was significantly different from that in the model group (P<0.05). Positive rate of PCNA expression aslo decreased significantly, showing statistical difference from the model group (P<0.05). Conclusion: TD inhibits tumor growth on nude mice modeled with MGC803, through down regulation of PCNA protein expression.