健骨颗粒对去卵巢骨质疏松模鼠骨组织结构的影响

目的 :观察健骨颗粒对去卵巢骨质疏松模鼠股骨颈超微结构的影响以及同期骨密度 (BMD)的改变 ,探讨该药药效及作用机制。材料和方法 :SD雌性大鼠去卵巢造模 ,随机分成健骨颗粒预防组和治疗组 ,并设生理盐水和骨松宝对照组。术后 2 4周处死 ,分别行骨密度检测及股骨颈骨粒透射电镜观察。结果 :去势 1 2周 ,骨细胞以吸收相和退变相为主 ,BMD明显降低。生理盐水组骨细胞多表现为退变相 ,骨细胞性溶骨更加明显 ,且出现破骨细胞性溶骨现象。健骨颗粒治疗组和骨松宝组多见成骨相骨细胞 ,成骨细胞活性加强 ,BMD明显升高。健骨颗粒预防组活跃的成骨细胞数量明显增加 ,可见成骨细胞成骨过程 ,BMD接近假手术组。结论 :健骨颗粒能有效防治骨质疏松的发生和发展。本文还就其作用机制进行了讨论。

EFFECT OF JIANGU GRANULA ON THE BONE TISSUE IN OVARIECTOMIZED RATS

Objective:To observe the effects of Jiangu granula on ultrastructure of neck of femur and the bone mineral density in ovariectomized rats,and to investigate its mechanism of action.Methods:Rats osteoporosis mode by ovary excision were randomly divided into Jiangu granula prevention group, Jiangu granula treating group,normal saline group and Gusongbao treating group.The animals were killed after 24 weeks of operation.Bone mineral density were measured and the ultrastructural of bone tissue of neck of femur were observed by transmission electron microscope.Results:After 12 weeks of ovariectomized,the osteocytes became most resorptive and degenerative phase osteocytes.Bone mineral density decreased.The osteocytes of normal saline group belonged to degenerative phase osteocytes,and osteocytic osteolysis was more remarkable.Osteoclast osteolysis also rised.The osteocytes of Jiangu granula group and Gusongbao group belonged to formative phase osteocytes.The activity of osteoblast was rising.Bone mineral density increased.Osteoblast numbers of Jiangu granula prevention group were higher.The osteogenetic process of osteoblast were observed.Bone mineral density approached the sham operated group level.Conclusion: Jiangu Granula can prevent and cure the occurrence and progress of osteoporosis.