姜黄素对二乙胺基亚硝胺诱发小鼠肝癌前病变的预防作用

目的:观察姜黄素对小鼠肝癌前病变的预防作用.方法:每周腹腔注射二乙基亚硝胺(DEN,95 mg·kg-1)建立小鼠肝癌前病变,每天灌胃姜黄素(120 mg·kg-1)或姜黄素与山药(3.03 g·kg-1)联合用药,连续10周(70 d);第71天眼球后静脉丛取血,检测血清肝功指标丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和谷胱甘肽-S-转移酶(GST),肝脏经灌注固定后用光学显微镜观察病理组织学变化.结果:模型组小鼠肝脏同时存在变质与修复性病变,增生肝细胞具有异型性;血清ALT:(195.57±91.56) U·L-1、AST:(39.68±24.20) U·L-1、GST:(42.31±8.19)U·L-1升高明显(P<0.01).姜黄素组肝损伤较轻,增生肝细胞异型性降低,ALT:(98.54±48.07) U·L-1、AST:(26.89±7.51) U·L-1、GST:(47.47±8.54) U·L-1下降明显(P<0.05),姜黄素联合山药组ALT:(82.75±38.54) U·L-1、AST:(30.32±7.80) U·L-1、GST:(24.61±7.58) U·L-1疗效优于单用(P<0.01).结论:姜黄素对诱导性小鼠肝癌前病变具有防治作用,姜黄素与山药联合用疗效更佳.

Preventive Effect of Curcumin on DEN-induced Hepatic Precancerous Lesions in Mice

Objective： To confirm the protective effect of curcumin on diethylnitrosamine（DEN）-induced hepatic precancerous lesions in mice.Method： The male mice were randomly divided into four groups.The hepatic precancerous lesions in mice were replicated with DEN（95 mg·kg-1） intraperitoneal administration once a week for 10 weeks,meanwhile curcumin（120.0 mg·kg-1） with or without Rhizoma Dioscoreae（3.03 g·kg-1） was administrated intragastrically once a day to protect the hepatic precancerous lesions.On the 71st day,the blood was collected to detect the hepatic injury biomarkers,and partial liver was fixed to observe the pathological changes.Result： Compared with those in the control mice,the hepatic precancerous lesions in the model mice were confirmed as the alterative and proliferative changes of liver,the proliferative hepatocytes were demonstrated as the atypia similar to hepatic carcinoma,the alterative changes were supported by the elevated activities of serum ALT（195.57±91.56） U·L-1,AST（39.68±24.20） U·L-1 and GST（42.31±8.19）U·L-1.The administration of curcumin relieved the hepatic precancerous lesions significantly（P0.01）,and the combination use of curcumin and Rhizoma Dioscoreae could enhance the effect（P0.05） in the treatment groups with ALT（82.75±38.54） U·L-1,AST（30.32±7.80） U·L-1and GST（24.61±7.58） U·L-1,respectively.Conclusion： Curcumin can protect the hepatic precancerous lesions in the mice attacked by diethylnitrosamine,and combination use of curcumin and Rhizoma Dioscoreae can be more effective.