| 体外培育牛黄抑制大鼠三叉神经节细胞电压依赖性钙通道电流参与镇痛机制 |
| |
| 引用本文： | 张韶辉,黄铁花,李璐璐,刘杨从. 体外培育牛黄抑制大鼠三叉神经节细胞电压依赖性钙通道电流参与镇痛机制[J]. 中国药师, 2012, 15(9): 1232-1234 |
| |
| 作者姓名： | 张韶辉 黄铁花 李璐璐 刘杨从 |
| |
| 作者单位： | 武汉市第一医院,武汉,430022 |
| |
| 摘 要： | 目的:研究体外培育牛黄(CBS)对大鼠三叉神经节(TRG)细胞电压依赖性钙通道电流(ICa)的影响,探讨牛黄镇痛作用的电生理机制。方法:在培养大鼠TRG细胞上采用全细胞膜片钳记录ICa。结果:CBS能够剂量依赖性地抑制TRG细胞电压依赖性钙通道电流,0.2,2,20μg.ml-1CBS可使钙电流幅值分别减少(30.3±4.7)%、(41.9±3.6)%和(56.7±6.8)%(n=6,P<0.01)。结论:CBS对钙电流的阻滞作用可能是其镇痛作用机制之一。
|
| 关 键 词： | 体外培育牛黄 膜片钳 电压依赖性钙通道电流 三叉神经元 |
| 收稿时间： | 2012-05-04 |
| 修稿时间： | 2012-06-18 |
Inhibition of Cultured Calculus Bovis Sativus on the Currents of Voltage-dependent Calcium Channel in Rat Trigeminal Ganglion Involved in the Mechanism of Analgesia |
| |
| Zhang Shaohui,Huang Tiehu,Li Lulu and Liu Yangcong. Inhibition of Cultured Calculus Bovis Sativus on the Currents of Voltage-dependent Calcium Channel in Rat Trigeminal Ganglion Involved in the Mechanism of Analgesia[J]. China Pharmacist, 2012, 15(9): 1232-1234 |
| |
| Authors: | Zhang Shaohui Huang Tiehu Li Lulu Liu Yangcong |
| |
| Affiliation: | (Wuhan NO.1 Hospital,Wuhan 430022,China) |
| |
| Abstract: | Objective： To investigate the effect of cultured calculus bovis sativus（CBS） on the currents of voltage-dependent calcium channel（ICa） in rat trigeminal ganglion（TRG） neurons in order to explore the electrophysiological mechanism of the analgesia of CBS.Method： The currents of voltage-dependent calcium channel on cultured TRG cells of rats were recorded by whole cell patch clamp.Result： CBS could dose-dependently inhibit ICa on TRG neurons,0.2,2 and 20 μg·ml-1 CBS could inhibit ICa by（30.3±4.7）%,（41.9±3.6）% and（56.7±6.8）%,respectively（n=6,P0.01）.Conclusion： The inhibitory effect of CBS on the calcium current may contribute to its analgesic effect. |
| |
| Keywords: | Calculus bovis sativus Patch clamp Currents of voltage-dependent calcium channel Trigeminal ganglion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药师》浏览原始摘要信息 |
|
点击此处可从《中国药师》下载免费的PDF全文 |
