HPLC法同时测定康复新液中尿嘧啶、次黄嘌呤及肌苷含量

目的:建立HPLC法同时测定康复新液中的尿嘧啶、次黄嘌呤及肌苷含量.方法:色谱柱:Wates SunfireTM C18柱(250 mm×4.6 mm,5 μm );甲醇-3%甲醇溶液(含0.07%醋酸)梯度洗脱;检测波长254 nm;进样量:10 μl.结果:尿嘧啶在4.92～34.44 μg·ml-1 (r=0.999 9),次黄嘌呤在12.43～87.06 μg·ml-1(r=0.999 9),肌苷在24.77～173.40μg·ml-1(r=0.999 9)的浓度范围内呈良好的线性关系.尿嘧啶的平均回收率为102.63%(RSD=1.78%);次黄嘌呤的平均回收率为99.13%(RSD=1.89%);肌苷的平均回收率为98.98%(RSD=1.33%).结论:该法操作简便、准确,重复性、稳定性好,可用于同时检测康复新液中尿嘧啶、次黄嘌呤及肌苷的含量.

Determination of Uracil, Hypoxanthine and Inosine in Kangfuxin Liquids by HPLC

Objective： To establish the method for the determination of uracil,hypoxanthine and inosine in Kangfuxin liquids by HPLC.Method： The separation was carried out on a SunFireTM C18 column（250 mm×4.6 mm,5 μm）.The mobile phase was methanol-3% methanol solution（containing 0.07% acetic acid） with gradient elution,the detection wavelength was 254 nm,and the injection volume was 10 μl.Result： The linear range of uracil was 4.92-34.44 μg·ml-1（r=0.999 9）,the linear range of hypoxanthine was 12.43-87.06 μg·ml-1（r=0.999 9）,and the linear range of inosine was 24.77-173.40 μg·ml-1（r=0.999 9）.The average recovery of uracil,hypoxanthine and inosine was 102.63%（RSD=1.78%）,99.13%（RSD=1.89%）and 98.98%（RSD=1.33%）,respectively.Conclusion： The method is convenient,accurate and steady,and suitable for the determination of uracil,hypoxanthine and inosine in Kangfuxin liquids.